重建膀胱感觉和运动神经支配大鼠模型的建立及鉴定  

To identify a rat model established by reconstruction of bladder sensory and motor innervation

作　　者：赵建国[1] 雷德桥 蒙德鹏[1] 侯春林[1] 林浩东[1] 宗海洋[1] 陈寅生[1] 蔡雨卫 

机构地区：[1]第二军医大学长征医院骨科,上海200003

出　　处：《山西医药杂志》2015年第10期1091-1094,共4页Shanxi Medical Journal

基　　金：国家自然科学基金(10JC1418300)

摘　　要：目的同时重建SD大鼠膀胱的运动和感觉神经支配,建立和鉴定动物模型,为进一步研究排尿中枢重塑及机制奠定基础。方法 45只雌性SD大鼠分为对照组(10只),神经根切断组(15只)和神经根吻合组(20只)。神经根切断组大鼠,将腰椎(L)4以下双侧脊神经前后根全部切断;神经根吻合组在切断脊神经根后,将双侧L4神经前后根与骶椎(S)1相应神经根吻合;对照组不做手术处理。术后6个月,取各组大鼠分别行尿流动力学检测、神经根电刺激、神经吻合口甲苯胺蓝染色、盆神经节注射荧光金神经示踪染色和膀胱湿质量测量。结果神经根吻合组大鼠膀胱最大容量、残余尿量及膀胱湿质量均小于神经根切断组而大于对照组;神经根吻合组大鼠最大排尿压与对照组差异无统计学意义(P>0.05),但大于神经根切断组(P<0.05)。吻合神经根行电刺激可于膀胱检测到压力变化。神经吻合口甲苯胺蓝染色可见神经通过率达到54%。盆神经节内注射荧光金后,神经根吻合组可见L4脊髓节段双侧灰质荧光金染色,神经根切断组和对照组未在相应脊髓节段检测到荧光金染色。结论从形态和功能角度证明成功建立同时重建膀胱感觉及运动神经支配的大鼠动物模型,为进一步研究排尿中枢重塑及机制奠定基础。Objective To establish and identify SD rat models whose bladder motor and sensory innervation was rebuilt.It laid the foundation for further study on micturition center remodeling and mechanisms.MethodsForty-five female SD rats were randomly divided into a control group（n =10）,rhizotomy group（n =15）and nerve root anastomosis group（n=20）.For rats in rhizotomy group,their spinal nerve roots following the L4 all around was cut.Rats in nerve root anastomosis group were treated as anastomosis of the bilateral L4 nerve root and S1 nerve root after cutting the spinal nerve roots.Rats in control group were treated without surgery.In six months after surgery,rats were taken into urodynamic testing,nerve root stimulation,nerve anastomosis toluidine blue staining,fluorescence gold pelvic ganglia nerve tracer dye and bladder wet weight measurements.ResultsRats′bladder maximum capacity,residual urine volume and bladder wet weight in nerve root anastomosis group were less than those in the rhizotomy group,but more than those in control group.Rats′maximum voiding pressure in nerve root anastomosis group was more than that in rhizotomy group,but there are no statistical differences between the control group and nerve root anastomosis group（P〉0.05）.Electrical stimulation to nerve root anastomosis line can be used to detect the pressure change in the bladder.Nerve through rate could be checked by toluidine staining nerve anastomosis.After injection of fluorescent gold in pelvic ganglia,gold fluorescence staining is visible in the L4 spinal cord gray matter in nerve root anastomosis group but not visible in rhizotomy group and in control group.Conclusion The SD Rat model,whose bladder was reconstructed while motor and sensory innervation was rebuilt successfully,was proved from form and function.It laid the foundation for further study on micturition center remodeling and mechanisms.

关 键 词：大鼠 Sprague-Dawley 尿流动力学 荧光金 神经吻合 神经重塑 神经示踪 神经根电刺激 

分 类 号：R-332[医药卫生]