肝细胞癌中趋化因子CXCL5及其受体CXCR2的表达及意义  

The expressions of chemokine CXCL5 and its receptor CXCR2 in HCC and the significance in the prognostic evaluation

作　　者：申九妹[1] 成元华[2] 郑启忠[1] 余梦懿 

机构地区：[1]厦门市中医院病理科,福建省361009 [2]贵阳医学院病理学教研室,550004

出　　处：《实用医学杂志》2015年第12期1936-1939,共4页The Journal of Practical Medicine

基　　金：贵阳市科学技术计划项目[编号:(2009)筑科农合同字第3-020号];贵州省科学技术基金项目[编号:黔科合丁字(2009)2187号]

摘　　要：目的:检测趋化因子5(C-X-C motif ligand 5,CXCL5)及其受体CXCR2在肝细胞癌(hepatocellular carcinoma,HCC)中的表达,探讨其与临床病理特征的关系及其在预后评估中的意义。方法 :采用免疫组化En Vision两步法检测51例HCC病例中CXCL5、CXCR2的表达,分析其与临床病理特征及微血管密度(microvessel density,MVD)的关系。结果 :(1)HCC中CXCL5、CXCR2蛋白高表达率分别为64.7%、68.6%,高于癌旁组织(17.6%、15.7%,P<0.05);CXCL5蛋白的表达与肿瘤大小、病理分级、TNM分期及血管浸润有关;CXCR2蛋白的表达与病理分级及血管浸润有关。(2)HCC中的MVD水平高于癌旁组织(P<0.001),与CXCL5、CXCR2的蛋白表达均呈正相关。(3)CXCL5、CXCR2蛋白高表达组,HCC复发率及转移率均较高。结论:CXCL5、CXCR2高表达可能促进了HCC的发生发展及HCC内微血管生成,可用于HCC的预后评估。Objective To detect the expressions of chemokine CXCL5 and its receptor CXCR2 in hepatocellular carcinoma （HCC）, investigate their relationships between CXCL5, CXCR2 and clinicopathologic features and probe into the significance in the evaluation of prognosis. Methods Immunohistochemical Envision method was utilized to detect the expressions of CXCL5 and CXCR2 in HCC , to explore their relationship between CXCL5, CXCR2 and clinicopathologic features and MVD in HCC. Results （1） The high expression rates of CXCL5 and CXCR2 protein was 64.7% and 68.6%, respectively, significantly higher than those in adjacent tissues of HCC（17.6%, 15.7%, P 〈 0.05）. The overexpression of CXCL5 protein had correlation with tumor size, differentiation, TNM stage and vascular invasion （P 〈 0.05） and the overexpression of CXCR2 protein did with tumor grade and vascular invasion （P 〈 0.05）. （2） The value of MVD was higher in HCC than that in the adjacent tissues of HCC （P 〈 0.05）, and had positive correlation with the expressions of CXCL5, and CXCR2 proteins. （3） The higher rates of recurrence and metastasis were in the groups of higher expression of CXCL5 and CXCR2 proteins （P 〈 0.05）. Conclusions The overexpressions of CXCL5 and CXCR2 may promote the occurrence and development of HCC as well as the neovasculation in HCC. Therefore, they can be used as markers to evaluate the prognosis of HCC.

关 键 词：癌  肝细胞 CXCL5 CXCR2 MVD 免疫组化 

分 类 号：R735.7[医药卫生—肿瘤]