银杏内生真菌的分离纯化及抑菌性的鉴定  

The Isolation and Identification of Antibacterial Activity of Endophytic Fungi from Ginkgo biloba

作　　者：裴冬丽[1] 张红岩[1,2] 韩汝豪 李成伟[3] 

机构地区：[1]商丘师范学院生命科学学院/植物与微生物互作河南省高校重点实验室,河南商丘476000 [2]商丘师范学院体育学院,河南商丘476000 [3]周口师范学院生命科学学院/植物遗传与分子育种重点实验室,河南周口466001

出　　处：《湖北农业科学》2015年第9期2116-2119,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31071807);河南省教育厅项目(13B210199)

摘　　要：采用组织分离法从银杏(Ginkgo biloba)健康组织中分离得到25株内生真菌,根据菌株在PDA培养基上的培养特征,5株被鉴定为链孢霉菌属、4株为酵母属、4株为曲霉属,3株为毛霉属、3株为青霉属,2株为根霉属,1株为简梗孢霉属,3株分离菌株不产孢子。测定25株内生真菌对3种受试指示菌的抑制作用,共筛选得到11株至少对一种指示菌的生长有抑制作用的菌株,对抑菌活性较强的菌株进行形态学和显微分析鉴定。25 endophytic fungi strains inhabited in Ginkgo biloba were isolated by the way of tissue isolation. Among the strains,there were five Neurospora, four Saccharomyces,four Aspergillus,three Mucor,three Penicillium,two Rhizopus,one Chromosporium and three sterile mycelia respectively. Antimicrobial activity of these 25 strains were investigated against three indicator bacteria. Eleven strains with antimicrobial activity were obtained. The strains with the better antimicrobial activity were identified according to its morphological and microscopic characteristics.

关 键 词：分离 鉴定 银杏(Ginkgo biloba) 内生真菌 抑菌性 

分 类 号：S664.3[农业科学—果树学]