肺组织特异性X蛋白在非小细胞肺癌中的表达及与肺癌微转移生物作用的关系  

The expression of LunX in non-small cell lung cancer and its role in the micrometastasis

作　　者：彭国庆[1] 刘文洲[2] 冼磊[2] 

机构地区：[1]广西科技大学第二附属医院外三科,广西柳州545006 [2]广西医科大学第一附属医院心胸外科,南宁530021

出　　处：《广东医学》2015年第12期1866-1869,共4页Guangdong Medical Journal

基　　金：广西自然科学基金资助项目(编号:桂科青0640043)

摘　　要：目的研究肺组织特异性X蛋白(Lun X)在非小细胞肺癌(NSCLC)患者中的表达,探讨Lun X与NSCLC患者肿瘤微转移的关系。方法收集54例NSCLC患者,转移组(n=34)为NSCLC并肺门纵隔淋巴结转移,非转移组(n=20)为NSCLC无肺门纵隔淋巴结转移患者,另选取同期胸部良性病变行手术患者21例为对照组。采用荧光定量聚合酶链式反应法(FQ-PCR)、蛋白印记法和免疫组织化法检测54例NSCLC患者癌组织、癌旁组织及淋巴结中Lun X基因及蛋白的表达。结果 FQ-PCR结果显示转移组患者癌组织、癌旁组织及癌转移淋巴结中Lun X mRNA表达量均明显高于非转移组(P<0.01);蛋白印记结果显示转移组癌组织及癌转移淋巴结中Lun X蛋白相对表达量较非转移组明显上调(P<0.01);免疫组织化学结果显示转移组癌组织及癌转移淋巴结Lun X蛋白表达量明显高于非转移组(P<0.01),两组癌旁组织在蛋白印迹及免疫组化表达上差异无统计学意义,而对照组肺组织及肺门纵隔淋巴结无Lun X mRNA及蛋白表达。结论 Lun X在NSCLC患者癌组织、癌旁组织及淋巴结中均有表达,其表达的上调可能参与NSCLC患者肿瘤微转移的发生发展过程。Objective To study the expression of LunX in human non - small cell lung cancer （NSCLC） tissue and its correlation to the micrometastasis. Methods A total of 54 patients with NSCLC were enrolled and divided into two groups ： the metastasis group （ metastasis in lymph nodes in the hilus of lung and the mediastinum） and the non - metastasis group. Twenty - one patients with pulmonary benign diseases were also enrolled as control group. Real - time fluorescence quantitative polymerase chain reaction （ FQ - PCR） , Western blotting and immunohistochemistry methods were used to analyze the expression of Lunx. Results According to FQ - PCR, the expression of LunX gene in the cancer tissues, adjacent tissues and lymph node of the metastasis group was significantly higher than non - metastasis group （ 1.65 ± 0. 18 vs. 0. 71 vs 0. 08, 1.14±0. 23 vs. 0. 57 ±0. 06, and 2. 57±0. 38 vs. 1.89 -＋0. 53, respectively, P 〈0. 01 ）. According to Western blotting, the expression of LunX channel in cancer tissues and lymph node of the metastasis group were significantly higher than non - metastasis group （ 1.67 ± 0.27 vs. 0. 84±0.27, and 3.12 ± 0. 31 vs, 1.97 ± 0.48, respectively, P 〈0. 01 ）. So was according to immunohistochemistry （4.04 ±0. 41 × 106 vs. 3.45 ±0. 43 × 106, and 3.95±0. 69 × 106 vs. 2.47 ± 0. 73× 106, respectively, P 〈 0. 01 ）. There was no LunX expression in non - cancer lung tissues. Conclusion LunX is expressed in the cancer tissues, adjacent tissues and lymph node in NSCLC patients, suggesting an important role in development of NSCLC.

关 键 词：非小细胞肺癌 肺组织特异性X蛋白 荧光定量PCR 蛋白印记 免疫组化 微转移 

分 类 号：R734.2[医药卫生—肿瘤]