γ-干扰素释放分析（T—SPOT．TB）在结核病诊断中的应用进展  

Advances in clinical application of interferon-gamma release assay in diagnosis of tuberculosis

作　　者：田祺[1] 谢永红[1] 徐淑凤[1] 刘丰遂[1] 赵靖[1] 刘秀娟[1] 刘菲菲[1] 卢宏志[1] 

机构地区：[1]秦皇岛市第一医院呼吸内科,066000

出　　处：《国际呼吸杂志》2015年第14期1090-1092,共3页International Journal of Respiration

基　　金：秦皇岛市科技研究与发展指导计划（2012023A127）

摘　　要：结核感染T细胞的快速血液检测——酶联免疫斑点实验T-SPOT．TB技术，是利用结核分枝杆菌感染外周血单个核细胞中存在的结核特异性T细胞进行检测。作为一项新型诊断方法，其发挥了显著的优越性。它具有较高的敏感性和特异性，已成为最敏感的检测方法之一。有许多文献表明用离体的酶联免疫斑点技术测定ESAT-6和CFP-10特异性T细胞较现有的结核菌素皮肤试验有明显的改进。越来越多的研究展示了T-SPOT．TB在肺内肺外结核病诊断中的临床应用价值。该文对T-SPOT．TB的临床应用进展作一简要综述。The enzyme-linked immunospot assay T-SPOT. TB （a rapid T cell-based blood test for tuberculosis infection） is based on detecting Mycobacterium tuberculosis specific T cells from peripheral blood mononuclear cells. As a hew diagnostic method, T-SPOT. TB has revealed a lot advantages. The high sensitivity and specificity make T-SPOT. TB be one of the most sensitive detection methods. In particular, there are substantial published evidences showing that the detection of ESAT-6 and CFP-10 specific T cells by the ex vivo enzyme-linked immunospot technique is a remarkable improvement over the tuberculin skin test. More and more researches have shown the clinical value of T-SPOT. TB in the diagnosis of pulmonary and extrapulmonary tuberculosis. This article makes a brief overview of the advances in the clinical application of T-SPOT. TB.

关 键 词：结核诊断 T-SPOT.TB Γ-干扰素释放试验 

分 类 号：R737.9[医药卫生—肿瘤]