响应面法优化五倍子总鞣质的提取工艺  

Optimization of Extraction Process of Total Tannin from Galla Chinensis by Response Surface Methodology

作　　者：陈丹妮[1,2,3] 秦昆明 陈林伟[1,2] 王彬 张金龙 蔡宝昌[1,2,3] 

机构地区：[1]南京中医药大学国家教育部中药炮制规范化及标准化工程研究中心,南京210023 [2]国家中医药管理局中药炮制标准重点研究室,南京210046 [3]南京海昌中药集团有限公司江苏省企业研究生工作站,南京210061

出　　处：《中国实验方剂学杂志》2015年第14期20-24,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省自然科学基金项目(BK20141093);国家中医药管理局公益性行业专项(201307008);江苏省科技支撑计划工业项目(BE2012011)

摘　　要：目的:优化五倍子总鞣质的提取工艺,为五倍子的深度开发和综合利用提供参考。方法:采用水浴加热浸提法,以没食子酸和浸出物得率为指标,在单因素试验基础上,选择提取次数、提取时间、液料比和提取温度为考察因素,采用BoxBehnken试验设计原理确定五倍子总鞣质的最佳提取工艺。结果:最佳提取工艺为加10.4倍量水于62℃水浴提取2次,每次125 min,没食子酸和浸出物得率分别为55.67%,65.11%,与理论预测值的相对偏差分别为3.30%,0.35%。结论:响应面法优选的五倍子总鞣质提取工艺方法简单、提取效率高、可预测性良好。Objective： To optimize extraction technology of total tannin from Galla Chinensis. Method： On the basis of single factor tests, water heating extraction process of total tannin from Galla Chinensis was optinfized by Box-Behnken design by taking yields of gallic acid and extract as indexes. Result： Optimum extraction technology of total tannin was extracted twice with 10.4 times the amount of water at 62 ℃ , 125 minutes of each time, yields of gallic acid and extract were 55.67% and 65.11% , compared with theoretical prediction values, their relative deviations were 3.30% and 0.35%, respectively. Conclusion： This optimized extraction technology of total tannin from Galla Chinensis is simple with high extraction efficiency and good predictability.

关 键 词：五倍子 总鞣质 没食子酸 水浴浸提法 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]