香附挥发油的生物活性及其GC-MS分析  

Biological Activity of Cyperi Rhizoma Volatile Oil and Its GC-MS Analysis

作　　者：张跃飞 李鑫[2] 孟宪生[2,3] 王帅[2,3] 包永睿[2,3] 崔亚玲[2] 

机构地区：[1]国家中成药工程技术研究中心,辽宁本溪117004 [2]辽宁中医药大学药学院,辽宁大连116600 [3]辽宁省组分中药工程技术研究中心,辽宁大连116600

出　　处：《中国实验方剂学杂志》2015年第14期32-35,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81241111);辽宁省高等学校优秀人才支持计划项目(LR2013044)

摘　　要：目的:采用体内半固体糊碳末推进法及体外MTT法证明香附挥发油促胃肠动力的生物活性,并揭示其发挥药效的物质基础。方法:采用半固体糊碳末推进法测定香附挥发油胃残留率及小肠推进率,MTT法测定香附挥发油对大鼠小肠平滑肌细胞的体外增殖率,同时采用GC-MS法解析发挥药效的挥发油成分组成。结果:香附挥发油中、高浓度的胃残留率及小肠推进率较空白对照组有显著性差异,对大鼠小肠平滑肌细胞均有一定的增殖促进作用,且表现出一定的量效关系;共鉴定出17种香附挥发油成分,主要包括α-香附酮,氧化石竹烯,2(1H)naphthalenone,3,5,6,7,8,8a-hexahydro-4,8a-dimethyl-6-(1-methylethenyl)等。结论:该实验揭示了香附挥发油促胃肠动力的物质基础主要为萜类,为其临床合理应用及进一步的研究开发提供合理依据。Objective： To verify the promoting gastrointestinal motility activity of Cyperi Rhizoma volatile oil using semi-solid paste method （in vivo） and MTT assay （ in vitro）. The chemical components of volatile oil were analyzed. Method： Stomach and small intestine propulsion rate of residual semi-solid paste of carbon propelling was used to determine the promoting gastrointestinal motility activity. MTT method was used to investigate the proliferation rate of small intestine smooth muscle cells in vitro. At the same time GC-MS method was used to analyze the components of volatile oil. Result： The rate of stomach and small intestine propulsion were significant differences between high concentration group and control group. Cyperi Rhizoma volatile oil with different concentration presents certain promoting effect to small intestine smooth muscle cells, and showed some concentration-response relationship. 17 ingredients were identified, containing a-cypcrone, caryophylene oxide, 2 （1H） -naphthalenone,3, 5, 6, 7, 8, 8a-hexahydro-4, 8a-dimethyl-6-（1-methylethenyl）, and so on. Conclusion： This study reveals the material base of Cyperi Rhizoma volatile oil promoting gastrointestinal motility is mainly terpenoids, which provide reasonable basis for its clinical application and further research and development.

关 键 词：香附 挥发油 胃肠动力 气相色谱-质谱 

分 类 号：R284.1[医药卫生—中药学]