胡黄连苷-Ⅱ原料药的定性与定量分析  

Qualitative and Quantitative Analysis of Active Pharmaceutical Ingredient Picroside-Ⅱ

作　　者：李鹏[1] 郑璐[1] 汪斌[1] 薛明[1] 陈炜伟[1] 姚仲青[1] 

机构地区：[1]扬子江药业集团有限公司中药研究院,江苏泰州225321

出　　处：《中国实验方剂学杂志》2015年第14期56-58,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的：对胡黄连苷-Ⅱ原料药进行结构鉴定,建立HPLC含量测定方法。方法：根据理化常数和光谱数据进行结构鉴定;采用反相高效液相色谱法,对胡黄连苷-Ⅱ原料药进行含量测定。结果：胡黄连苷-Ⅱ原料药结构鉴定表明其成分为6-香草酰基梓醇,即胡黄连苷-Ⅱ;HPLC测定胡黄连苷-Ⅱ在0.315~5.05μg进样量与峰面积线性关系良好,平均加样回收率为98.09%,RSD 1.6%,3批胡黄连苷-Ⅱ原料药质量分数分别为101.53%,101.50%,102.25%。结论：胡黄连苷-Ⅱ原料药含量较高,所建立含量测定方法简便可行,重复性好,可用于胡黄连苷-Ⅱ原料药的质量控制。Objective： To identify the structure of picroside-Ⅱ and establish an HPLC method for determining the content of picroside-Ⅱ . Method： The structure was elucidated by its physiochemical characteristics and spectral analysis. The content of picroside-Ⅱ was determined by HPLC. Result： The structure was elucidated as 6-vanilloyl catalpol （ picroside- Ⅱ ）. The linear range of picroside- Ⅱ was within 0. 315-5.05 txg. The average recovery was 98.09% , with RSD 1.6%. The three batches of active pharmaceutical ingredient picroside-Ⅱ were 101.53%, 101.50%, and 102.25%, respectively. Conclusion： The method is simple and specific, and can be used for control of picroside- Ⅱ.

关 键 词：胡黄连苷-Ⅱ 原料药 定量 定性 

分 类 号：R284.1[医药卫生—中药学]