双齿型L-脯氨酸手性配体交换色谱固定相的合成及对氯苯那敏的拆分  

Synthesis of Bidentate L-Proline Chiral Ligand-exchange Chromatography Stationary Phase and the Separation of Chlorpheniramine

作　　者：黄德友[1] 李杨[1] 李连杰[1] 蒋登高[1] 黄明贤[1] 

机构地区：[1]郑州大学化工与能源学院,河南郑州450001

出　　处：《化学试剂》2015年第7期621-623,638,共4页Chemical Reagents

摘　　要：以3-氨丙基三乙氧基硅烷和三聚氯氰为连接臂合成了一种新型的双齿型L-脯氨酸手性配体交换色谱固定相,并利用元素分析进行了表征。本手性配体交换色谱固定相成功地拆分了手性药物氯苯那敏,并详细考察了流动相p H、中心金属离子浓度和缓冲溶液浓度以及柱温等对拆分效果的影响,得到了较佳拆分条件。在较佳拆分条件下,氯苯那敏的分离度可达2.87。同时对分离机理进行了简单的探讨。A new bonded chiral ligand-exchange chromatography stationary phase has been synthesized using bidentate L-proline and cyanuric chloride linker on aminopropyl-silica gel and was confirmed by elemental analysis. The chiral ligand-exchange chromatography stationary phase has successfully resolved the chiral drug of chlorpheniramine. The effect of resolution on the concentration of the metal ions and the buffer solution,the p H of the mobile phase and the temperature of the column were systematically investigated in order to obtain the best separation conditions. Under optimum conditions,the separation of chlorpheniramine reach2. 87. Furthermore,a plausible chiral recognition mechanism for resolution of enantiomers was proposed.

关 键 词：配体交换色谱固定相 氯苯那敏 拆分条件 分离机理 

分 类 号：O658[理学—分析化学]