新疆乌苏地区棉花黄萎病菌分离鉴定和致病力分析  

Identification and Pathogenicity Analysis of Cotton Verticillium dahliae Strains from Wusu of Xinjiang

作　　者：孔德真 黎玉顺 裴娟[1] 庞学兵 王爱英[1] 

机构地区：[1]新疆石河子大学生命科学学院,新疆石河子832000

出　　处：《中国农学通报》2015年第20期61-66,共6页Chinese Agricultural Science Bulletin

基　　金：转基因专项"转基因棉花环境安全评价技术"(2014ZX08011-002)

摘　　要：为了研究新疆乌苏地区棉花黄萎病菌的致病型和群体间的变异性,从该地区不同棉株上分离8株病原菌,通过对病原菌的菌落培养形态、菌丝和孢子的显微结构、对寄主的致病性、ITS序列的克隆、菌株间的系统进化及营养亲和性分析等方面进行了研究。结果表明:分离的病原菌均属于非落叶型棉花黄萎病菌(Verticillium dahliae),为大丽轮枝菌;系统进化树显示8株菌在系统进化上属于2个不同的进化方式;8株黄萎病菌在相同的寄主上致病性存在差异,Vd-1菌株致病力最强,Vd-34致病力最弱;不同致病力的菌株之间的营养亲和性分为2个营养亲和群(VCGs)。新疆乌苏地区棉花黄萎病的致病菌是大丽轮枝菌,病原菌在进化上差异显著。In order to study the pathotype population variation and pathogenicity of cotton Verticillium dahliae in Wusu of Xinjiang, eight cotton V.dahliae strains from different cotton plants in this area were used as thematerials for analyzing colony morphology, microscopic structure, pathogenicity, internal transcribed spacer（ITS） sequence, evolution and vegetative compatibility（VCG）. The analysis of colony morphology, microscopicstructure and ITS sequence indicated that the eight collected strains belonged to non- defoliating type V.dahliae. The constructed phylogenetic tree indicated that strain evolution could be divided into two differentways. Eight strains had differences in pathogenicity, Vd-1 strain showed the highest pathogenicity, while Vd-34 strain showed the lowest pathogenicity. The vegetative compatibility analysis indicated that the strains withdifferent pathogenicity belonged to two vegetative compatibility groups（VCGs）. Cotton verticillium wilt wascaused by Verticillium dahliae in Wusu of Xinjiang, pathogens had significant differences in evolution.

关 键 词：黄萎病菌 ITS序列 系统进化树 营养亲和性 致病力 

分 类 号：S435.61[农业科学—农业昆虫与害虫防治]