PBMCs生成IL-10、IL-12的水平与晚期NSCLC患者感染的相关性研究  

The correlation study between IL-10 and IL-12 levels from PBMCs and infection in advanced NSCLC patients

作　　者：杜琨[1] 李世荣[2] 王超[2] 

机构地区：[1]首都医科大学附属北京妇产医院医务部,北京100026 [2]首都医科大学附属北京友谊医院感染内科,北京100050

出　　处：《临床和实验医学杂志》2015年第13期1065-1068,共4页Journal of Clinical and Experimental Medicine

基　　金：北京市卫生局首都医学发展科研基金资助项目(编号2009-1034)

摘　　要：目的明确合并严重感染的晚期非小细胞肺癌(NSCLC)患者外周血单核细胞(PBMCs)生成白介素10(IL-10)及白介素12(IL-12)水平的变化是否为NSCLC患者发生感染或感染后恢复的预测因素。方法纳入45例的晚期NSCLC患者,其中合并初发感染NSCLC患者组(IIG组)15例、感染恢复1月后的NSCLC患者(RIG组)15例、初次化疗前无感染的NSCLC患者(NIG组)15例,另选同期体检者15例设为健康对照组(HC组)。四组均抽取经肝素抗凝的外周血3 ml,测定PBMCs产生细胞因子IL-10与IL-12的含量。PBMCs培养基上清液中细胞因子IL-10与IL-12浓度通过ELISA的方法测定。结果四组PBMCs产生的IL-10的水平中位数无显著差异(P>0.05)。IL-12的表达水平在IIG、RIG和NIG组均高于HC组,差异有统计学意义(P<0.01);虽然RIG组的IL-12水平低于IIG组,但仍高于HC组,差异具有统计学意义(P=0.01)。结论 PBMCs生成的IL-12水平不仅是肿瘤负荷存在的标志,也是一个NSCLC患者合并严重感染或感染后恢复的预测因素。Objective To assess if the variation of IL- 10 and IL- 12 generated by peripheral blood mononuclear cells（ PBMCs） is a predictive factor for the severe infection in advanced non- small cell lung cancer（ NSCLC） patients. Methods Forty- five cases of advanced NSCLC were included in the study. The patients were divided into initial infection group（ n = 15）,one month after recovery from infection group（ n = 15） and non- infection group（ n = 15）. Fifteen healthy subjects were in control group. Peripheral blood with heparin anticoagulant was obtained from the four groups,and IL- 10 and IL- 12 production in the culture supernatant of PBMCs was determined by ELISA. Analysis of variance was used to analyze the difference among groups and P〈0. 05 was believed to have statistical significance. Results The median level of IL- 10 exhibited no difference among the 4 groups（ P〉0. 05）. IL- 12 levels in the initial infection group,recovery from infection group and non- infection group were higher compared to the healthy control group（ P〈0. 01）. Although IL- 12 level in recovery from infection group was lower than initial infection group,the difference compared to the healthy control group was still statistically significant（ P = 0. 01）. Conclusion IL- 12 generated by PBMCs is not only a sign of tumor load,but also is a prediction factor for severe infection or recovery from infection in NSCLC patients.

关 键 词：非小细胞肺癌 感染 外周血单核细胞 白介素10 白介素12 

分 类 号：R734.2[医药卫生—肿瘤]