小白菊内酯促进CD34^+CD38^-白血病干细胞凋亡的表面粘附分子CXCR4机制研究  

The involvement of CXCR4 and surface adhesion factor in parthenolide induced leukemia stem cell apoptosis

作　　者：章志福[1] 

机构地区：[1]广州市花都区人民医院内六科,广东广州510800

出　　处：《临床和实验医学杂志》2015年第14期1155-1158,共4页Journal of Clinical and Experimental Medicine

基　　金：花都区人民医院医疗卫生项目(课题序号:14-HDWS-018);项目名称:小白菊内酯干预白血病干细胞粘附骨髓基质细胞的作用及机制

摘　　要：目的探讨小白菊内酯促进CD34+CD38-白血病干细胞凋亡的表面粘附分子CXCR4机制,为相关临床治疗提供参考。方法免疫磁珠法分离急性髓系白血病患者骨髓获得CD34+CD38-白血病干细胞,不同剂量小白菊内酯干预后分析各组细胞CXCR4蛋白表达及CD62L、CD44和CD49d的表达量及荧光强度。结果小白菊内酯可明显增强Caspase细胞凋亡蛋白家族表达,降低CXCR4蛋白表达和CD62L、CD44和CD49d表面荧光强度,且与白血病干细胞组相比具有显著的统计学差异性(P<0.05,P<0.01);小白菊内酯的促凋亡作用呈现出剂量依从性。结论小白菊内酯主要通过抑制白血病干细胞细胞表面相关粘附分子CD62L、CD44和CD49d及CXCR4蛋白的表达发挥促白血病干细胞凋亡作用。Objective The aim of the present research is to explore the involvement of CXCR4 and surface adhesion factor in parthenolide induced leukemia stem cell apoptosis. Methods Leukemia stem cell was isolated by immunomagnetic beads and the expression of CXCR4 as well as CD62L, CD44 and CD49d was detected after medication of parthenolide by western blotting or Real time PCR. Results Parthenohde enhanced the expression of caspases and decreased the expression of CD62L, CD44 and CD49d in a dose dependent manner. Conclusion Parthenolide exerts its apoptosis inducement function in leukemia stem cell by decreasing the expression of CXCR4 and CD62L, CD44 as well as CD49d.

关 键 词：白血病干细胞 小白菊内酯 表面粘附分子 细胞凋亡 剂量依从性 

分 类 号：R733.7[医药卫生—肿瘤]