白细胞介素2联合氩氦冷冻消融对中晚期肝癌免疫状态影响的研究  

Effects of argon-helium cryoablation combined with Interleukin 2 on the immune status of patients with advanced hepatocellular carcinoma

作　　者：叶伟东[1] 涂建飞[1] 纪建松[1] 虞作春[1] 

机构地区：[1]浙江省丽水市中心医院感染科,323000

出　　处：《中华全科医学》2015年第9期1402-1404,共3页Chinese Journal of General Practice

基　　金：浙江省科技厅重大科技专项项目(2013C03010);浙江省丽水市科技局项目(2012JYZB20)

摘　　要：目的通过白细胞介素2针联合氩氦冷冻消融治疗,评估联合治疗对进展期肝癌患者的免疫状态的价值。方法 1将62例临床诊断或者病理确诊原发性肝癌患者的临床资料,根据治疗方式分为冷冻组(冷冻消融)30例,联合组(冷冻消融+白介素2针)32例,2组患者性别、年龄、病理比较差异无统计学意义,有可比性。2通过治疗前、后外周血T淋巴细胞亚群测定评价。结果冷冻组治疗前CD4、NK细胞水平及CD4/CD8分别为(23.22±4.75)%、(9.50±2.28)%、0.85±0.12;治疗后2周分别为(37.56±3.98)%、(17.16±2.91)%、1.25±0.13;治疗后4周分别为(36.07±5.74)%、(16.97±2.47)%、1.20±0.17。联合组治疗前CD4、NK细胞水平及CD4/CD8分别为(24.15±4.37)%、(10.05±2.39)%、0.86±0.15;治疗后2周分别为(39.24±5.13)%、(17.35±3.17)%、1.26±0.15;治疗后4周分别为(53.86±6.91)%、(25.56±3.18)%、1.57±0.21。2组较治疗前相比差异均具有统计学意义(P<0.001);但联合组在治疗2周后CD4、NK细胞水平及CD4/CD8值略高于冷冻组,但差异无统计学意义(P>0.05)。结论从T淋巴细胞亚群指标分析,白细胞介素2针联合氩氦刀治疗组中T淋巴细胞亚群变化好于单纯氩氦刀治疗组。Objective To observe the effects of argon-helium cryoablation combined with Interleukin 2 （IL-2） on the im- mune status of patients with advanced hepatoee]lular carcinoma. Methods The clinical data of 62 cases of hepatocellular carcinoma confirmed by clinical treatment or pathology were analyzed retrospectively. The patients were divided into cryoablation group and combined group with 22 cases in each group according to the operation method. There was no significant difference between the two groups in terms of basic information. The indicators of T lymphocyte subsets in peripheral blood were measured before and after the treatment. Results The levels of CD4 and NK and CD4/CD8 in the cryoablation group were （ 23.22 ~ 4.75 ） %, （ 9. 50 ± 2. 28 ） %, 0. 85 ±0. 12 before the treatment ; （ 37. 56 ± 3.98 ） %, （ 17. 16 ± 2. 91 ） %, 1.25 ± 0. 13 two weeks after the treatment ; （ 36.07 ±5.74） %, （ 16. 97 ± 2.47 ） %, 1.20 ± 0. 17 four weeks af- ter the treatment, respectively. The levels of CD4, NK and CD4/CD8 in the combined group were （24. 15± 4. 37）%, （ 10. 05 ± 2. 39） % ,0. 86 ± 0. 15 ; （ 39. 24 ± 5.13 ） %, （ 17. 35± 3.17 ） %, 1.26 ± 0. 15 two weeks after the treatment ; （53.86 ± 6. 91 ） %, （25. 56 ± 3. 18 ） % , 1.57 ±0. 21 four weeks after the treatment, respectively. CD4 and NK level and CD4/CD8 value in both groups after the treatment were higher than that before the treatment, there was a significant difference（P 〈0. 001 ）. CD4 and NK cell level and CD4/CD8 value in the combined group two weeks after the treatment were slightly higher than the cryoablation group, but no significant difference was found （ P 〉 0.05 ）. Conclusion From the indicators of T lymphocyte subsets, the changes in the combined group （ argon-helium cryoablation combined with Interleukin 2） are better than that in the cryoablation group.

关 键 词：原发性肝癌 免疫 氩氦消融 

分 类 号：R735.7[医药卫生—肿瘤] R730.3[医药卫生—临床医学]