何首乌50%乙醇洗脱部位中单体化合物的分离及纯化  

Isolation and purification of 50% ethanol extract of Polygonum multiflorum

作　　者：殷永佳 周满如[1] 李近[1] 郑雅元[1] 陈文双[2] 曾小斌[2] 刘钰瑜[1] 

机构地区：[1]广东医学院药理学教研室,广东湛江524023 [2]广东医学院天然药物研究与开发重点实验室,广东湛江524023

出　　处：《广东医学院学报》2015年第1期45-48,51,共5页Journal of Guangdong Medical College

基　　金：国家自然科学基金课题(No.2060045);广东省科技计划项目(No.ZK0542);广东医学院药学院本科毕业论文选题与质量保障工程项目(No.广医药教改〔2013〕4-13)

摘　　要：目的从何首乌50%乙醇洗脱部位中分离得到单体化合物。方法何首乌乙醇粗提液用旋转蒸发仪浓缩,经D101大孔吸附树脂、硅胶柱子、凝胶柱子纯化,利用物理化学方法和核磁共振方法对获得化合物进行结构鉴定。结果从何首乌50%乙醇洗脱部位中分离并鉴定出2个单体化合物:β-胡萝卜苷及Emodin-1-O-β-D-glucopyranoside。结论该方法简便、快速、灵敏度高,可用于何首乌单体化合物的分离。Objective To extract and isolate monomeric compounds from 50% ethanol extracts of Polygonum multiflorum. Methods Crude ethanol extract of Polygonum multiflorum was concentrated by rotary evaporator, and then purified by D101 macroporous resin, silica gel and Sephadex LH-20 columns. The componds were identified using physicochemical methods and nuclear magnetic resonance. Results Two monomeric compounds β-daucosterol and emodin-1-O-β-D-glucopyranoside were isolated from 50% ethanol extracts of Polygonum multiflorum. Conclusion This method is simple, rapid and sensitive for isolation of Polygonum multiflorum.

关 键 词：何首乌 分离 单体化合物 

分 类 号：R914.15[医药卫生—药物化学]