氢自养反硝化细菌SY6的反硝化特性研究  

Characterization of Denitrification of a Hydrogen Autotrophic Denitrification Bacterium SY6

作　　者：苏俊峰[1,2] 张凯[1] 黄廷林[1] 黄文斌[1] 卢金锁[1] 郭琳[1] 杨少斐 

机构地区：[1]西安建筑科技大学环境与市政工程学院,陕西西安710055 [2]清华大学国家环境保护环境微生物利用与安全控制重点实验室,北京100084

出　　处：《应用基础与工程科学学报》2015年第3期493-498,共6页Journal of Basic Science and Engineering

基　　金：国家科技支撑计划(2012BAC04B02);国家环境保护环境微生物利用与安全控制重点实验室开放基金(MARC2012D001);陕西省教育厅专项科研计划项目(12JK0896);陕西省教育厅重点实验室科学研究计划项目(12JS051)

摘　　要：从水库底泥中分离出1株氢自养反硝化细菌SY6.以氢自养反硝化菌株SY6作为研究对象,分析了氢气作为电子供体时,氢自养反硝化细菌SY6生物脱氮途径及生长增殖规律,考察了不同环境因子对菌株SY6生物脱氮性能的影响.结果表明,30℃时菌株反硝化效率最高,此时NO-3的去除率达到100%;在p H值为6—7的中性偏酸环境,菌株反硝化效果最好,NO-3的去除率为100%.不同的C/N对反硝化效果的影响很小,以Na HCO3作为碳源反硝化效果优于以CO2作为碳源.A hydrogen autotrophic denitrifying bacterium SY6 isolated from sediment was used to investigate the biodenitrification pathway,growth behavior and the effect of the environmental factors on the denitrification efficiency under hydrogen as sole electron donor condition. The results showed that the best denitrification efficiency was achieved when temperature was 30℃,the nitrate nitrogen removal rate was 100%. The fastest degradation rate of nitrate was achieved when p H was 6. 0 to 7. 0,the nitrate nitrogen removal rate was 100%. Different carbon-nitrogen ratio did not influence the efficiency of denitrification. Bicarbonate as the only inorganic carbon source was better than that only with carbon dioxide as carbon sources.

关 键 词：氢自养反硝化 生物脱氮 温度 碳源 

分 类 号：X172[环境科学与工程—环境科学]