黑果枸杞内生真菌RER4化学成分研究  

Chemical constituents of endophytic fungi RER4 fromLycium ruthenicum

作　　者：杨秀芳[1] 王鹏飞[1] 马养民[1] 

机构地区：[1]陕西科技大学化学与化工学院教育部轻化工助剂化学与技术重点实验室,陕西西安710021

出　　处：《陕西科技大学学报（自然科学版）》2015年第4期121-126,共6页Journal of Shaanxi University of Science & Technology

基　　金：教育部高等学校博士学科点专项基金项目(20116125110001)

摘　　要：根据形态学特征和ITS序列对分离自黑果枸杞根部的内生真菌RER4进行鉴定,结果该真菌鉴定为米曲霉(Aspergillus oryzae).采用大米固体培养基对RER4进行发酵,利用多种色谱方法从乙酸乙酯提取物中分离纯化得到8个化合物,借助ESI-MS、NMR等波谱技术鉴定其结构分别为:麦角甾醇(1)、尿黑酸内酯(2)、6-柠檬酸甲酯(3)、琥珀酸(4)、1,5-柠檬酸二甲酯(5)、赤薛醇(6)、甘露醇(7)、阿拉伯糖醇(8).其中化合物尿黑酸内酯首次从米曲霉发酵物中分离得到.The fungus strain RER4 isolated from the root of Lycium ruthenicum.Was identified as Aspergillus oryzae by its morphological characteristics and internal transcrised spacer（ITS）sequence analyses.Solid state fermentation was used to culture the endophytic fungi RER4 on the rice medium,8compounds were isolated and purified from the eyhyl acetate extract of endophytic fungi RER4 by various chromatographic methods and their structures were ergosterol,5-hydroxy-benzofuran-2（3H）-one,3-hydroxy-3-（methoxycarb onyl）pentanedi-oic acid,succinic acid,2-hydroxy-4-methoxy-2-（2-meth oxy-2-oxoethyl）-4-oxobutan-oic acid,erythritol,mannitol,adonitol by ESI-MS and NMR.Among compound 5-hydroxybenzofuran-2（3H）-one was firstly isolated from the medium of Aspergillus oryzae.

关 键 词：黑果枸杞 内生真菌 米曲霉 化学成分 结构鉴定 

分 类 号：R932[医药卫生—生药学]