PI3K/AKT信号通路在乳腺癌中的表达及其意义  

Expression of PI3K/AKT signal pathway in breast cancer and the clinical significance

作　　者：张刚[1] 李中[1] 林晓萌[1] 张军华[1] 崔勇[1] 赵醒[2] 

机构地区：[1]河北大学附属医院肿瘤外科,河北保定071000 [2]承德医学院附属医院病理科

出　　处：《中国妇幼保健》2015年第20期3475-3478,共4页Maternal and Child Health Care of China

基　　金：河北省卫生厅计划指导项目〔ZD20140360〕

摘　　要：目的探讨人乳腺癌及正常乳腺组织中P-AKT的表达及其意义,为乳腺癌的早期临床诊断和治疗提供理论和实验依据。方法采用Western blot及RT-PCR方法检测100份乳腺癌组织标本和30份正常乳腺组织标本中P-AKT蛋白及mRNA的表达情况,并进一步探讨其与乳腺癌临床病理指标的相关性。结果 P-AKT蛋白及mRNA在乳腺癌中的表达水平高于在正常乳腺组织中的表达水平,且P-AKT蛋白及mRNA的表达与临床分期相关(P<0.05),而与年龄、肿瘤直径、淋巴结转移和组织学分级无关(P>0.05)。结论 PI3K/AKT信号通路可能参与乳腺癌的发生发展,在癌变过程中起着重要作用。Objective：To explore the expressions of p-AKT in human breast cancer and normal breast tissue and the significance,provide theoretical and experimental basis for early clinical diagnosis and treatment of breast cancer.Methods：Western blot and RT-PCR were used to detect the expressions of p-AKT protein and mRNA in 100 cases with breast cancer and 30 cases with normal breast tissue,its correlation with clinicopathological indexes of breast cancer was further researched.Results：The expression levels of p-AKT protein and mRNA in breast cancer were statistically significantly higher than those in normal breast tissue.The expressions of p-AKT protein and mRNA were correlated with clinical staging（P〈0.05）,but it was not correlated with age,diameter of breast cancer,lymph node metastasis,and histological grading（P〈0.05）.Conclusion：PI3 K / AKT signal pathway may be involved in oncogenesis and development of breast cancer,which plays an important role in the course of cancerization.

关 键 词：乳腺癌 P-AKT WESTERN BLOT RT-PCR 

分 类 号：R737.9[医药卫生—肿瘤]