电感耦合等离子体发射光谱法测定深圳市母乳中钙和磷  

Determination of calcium and phosphorus in human milk in Shenzhen City by inductively coupled plasma optical emission spectrometer

作　　者：姜杰[1] 张慧敏[1] 李胜浓[1] 蒋友胜[1] 周健[1] 林凯[1] 丘红梅[1] 张建清[1] 

机构地区：[1]深圳市疾病预防控制中心,广东深圳518055

出　　处：《实用预防医学》2015年第8期915-917,共3页Practical Preventive Medicine

基　　金：国家科技部863项目(编号:2011AA060605);深圳市科技计划重点项目(编号:201101016);深圳市科创委项目(编号:JCYJ20130329103949643)

摘　　要：目的建立电感耦合等离子体发射光谱测定母乳中钙磷含量的方法，并对深圳市当地母乳钙、磷两种元素的含量进行测定。方法于2011—2013年收集深圳市产后1～2个月产妇的母乳样品共221例，样品冻干保存，经硝酸高压消解后采用电感耦合等离子体发射光谱法（ICP一0Es）测定母乳中钙和磷的含量。结果钙检出限0．09roe,／kg，磷检出限0．03me．／kg；各元素线性良好，相关系数R〉0．9999；钙测定精密度1．3％～2．5％，磷测定精密度1．6％～2．1％；国家标准参考物质的分析结果在证书值范围内；深圳市母乳中钙含量187．6～449．6mg／kg，平均值为294．5me,／kg，磷含量84．9--230．7me．／kg，平均值为157．7me．／kg，钙磷比1．1～3．7，平均值为1．9。结论电感耦合等离子体发射光谱法灵敏、准确可靠，可用于母乳中钙磷元素的同时检测。深圳市母乳中钙元素的平均含量在WHO提供的参考值范围内，磷元素的平均含量略高于WHO提供的参考值范围。Objective To develop a method for determination of calcium （Ca） and phosphorus （P）in human milk, and to in- vestigate the contents of Ca and P in human milk in Shenzhen City. Methods A total of 221 human milk （ 1 - 2 months postpartum） samples were collected and preserved after lyophilization in Shenzhen City during the period of 2011 - 2013. After high- pressure digestion by HNO3 in a closed - vessel, the dements of Ca and P were simultaneously determined by inductively coupled plasma optical emission spectrometer （ICP - OES）. Results The detection limits of Ca and P were 0.09 mg/kg and 0.03 mg/kg respectively. The correlation coefficients were both ~0.9999, with a good linearity. The relative standard deviation of Ca and P ranged from 1.3 % to 2.5 % and from 1.6 % to 2.1% respectively. The detection results of international reference standard materials for Ca and P both fell in the certificate value ranges. The level of Ca in human milk in Shenzhen City ranged from 187.6 mg/kg to 449.6 mg/kg, and the average concentration was 294.5 mg/kg. The level of P ranged from 84.9 m_g/kg to 230.7 mg/kg, and the average concentration was 157.7 mg/kg. The ratio of Ca and P ranged from 1.1 to 3.7, with the average value of 1.9. Conclusions The inductively coupled plasma optical emission spectrometer （ICP- OES） has a high accuracy and good reproducibility, which can be used to simultaneously determine the elements of Ca and P in human milk. The average content of Ca in human milk in Shenzhen is within the current reference ranges provided by the World Health Organization （WHO）, while the average content of P slightly exceeds the current reference ranges provided by the WHO.

关 键 词：母乳 钙 磷 电感耦合等离子体光谱法(ICP—OES) 

分 类 号：R15[医药卫生—营养与食品卫生学]