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机构地区:[1]四川农业大学小麦研究所,成都611130 [2]云南农业大学农学与生物技术学院,昆明650201 [3]保山学院资源环境学院,保山678000 [4]保山市种子管理站,保山678000
出 处:《中国粮油学报》2015年第7期7-11,共5页Journal of the Chinese Cereals and Oils Association
基 金:国家自然科学基金(31000712)
摘 要:为研究Wx基因的缺失对小麦淀粉生物合成的影响,本研究以8个Wx小麦近等基因系为材料,在灌浆期的10、20、30和40d取籽粒测定直链淀粉、支链淀粉、总淀粉的含量及合成速度。结果表明:直链淀粉、支链淀粉和总淀粉含量及积累速率在花后逐渐增加,以花后10~20d最大,此后逐渐降低。3个Wx基因对直链淀粉的含量及积累速率的影响依次为Wx—B1〉Wx—A1〉Wx—DI;小麦Wx基因型对直链淀粉的合成及积累速率影响较大,而对支链淀粉的影响较小,其对总淀粉含量及其积累的影响主要通过直链淀粉进行。In order to research the effect of absence of Wx gene on wheat starch biosynthesis, eight Wx near - isogenic wheat lines were used as materials. Amylose, amylopectin, total starch content and synthesis speed were de- termined during the grain filling period of 10, 20, 30 and 40 days. The results showed that the contents and accumu- lation rates of amylose, amylopectin and total starch increased after flowering. These elements reached the maximum value 10 -20 days after flowering, and then decreased gradually. The effect of 3 Wx genes on the content and accu- mulation rate of amylose was Wx - B1 〉 Wx - A1 〉 Wx - D1. The Wx genotypes showed a significant influence on starch biosynthesis and accumulation through synthesis and accumulation of amylase. While Wx genotypes did not in- volve in the biosynthesis of amylopectin. The effect of Wx genotypes on total starch biosynthesis and accumulation could be mainly influenced through amylose.
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