HPV E6/E7 mRNA检测用于ASCUS患者诊断分流的临床研究  

The role of human papillomavirus E6/E7 mRNA testing in triage of patients with ASCUS

作　　者：潘嫱微 陈育梅[1,2] 潘嘉佳 吕杰强[3] 

机构地区：[1]温州医科大学温州市第三临床学院 [2]温州市人民医院妇产科,325000 [3]温州医科大学附属第二医院妇产科

出　　处：《中国计划生育学杂志》2015年第7期471-473,共3页Chinese Journal of Family Planning

摘　　要：目的:探讨人乳头瘤病毒(HPV)E6/E7mRNA检测用于未明确诊断意义的不典型鳞状上皮细胞(ASCUS)诊断结果的判定价值。方法:对160例薄层液基细胞学检查诊断为ASCUS者进行HPV E6/E7mRNA和高危型HPV(HR-HPV)DNA检测,结合病理学诊断资料进行统计学分析。结果:160例中病理结果为宫颈上皮内瘤变Ⅱ级(CINⅡ)及以上级别者的HPV E6/E7mRNA阳性率明显高于CINⅡ以下级别者,差异有统计学意义(P<0.05),但与HR-HPV DNA检测结果无统计学差异。HPV E6/E7 mRNA检测CINII及以上级别的灵敏度(69.2%)与HR-HPV DNA检测相比无统计学差异(P>0.05);特异度(73.9%)高于HR-HPV DNA检测(61.9%),差异有统计学意义(P<0.05)。结论:HPV E6/E7 mRNA检测可作为ASCUS者是否需要进行阴道镜检查的一项指标依据。Objective：To investigate the clinical value of human papillomavirus（HPV）E6/E7 mRNA testing in patients with atypical squamous cell of undetermined significance（ASCUS）.Methods：A total of 160 patients with ASCUS cytology had colposcopy/biopsy,HPVE6/E7 mRNA and high-risk（HR）HPV DNA testing.The testing results combined with pathological diagnosis were analyzed.Results：The positive rates of HPV E6/E7 mRNA for cervical intraepithelial neoplasia（CIN）II or worse were significantly higher those of CIN I or normal（P〈0.05）,and the results were similar with those of HR-HPV DNA testing.The sensitivity of HPV E6/E7 mRNA for detecting CINII or worse（73.9%）was similar with that of HR-HPV DNA testing（P〉0.05）,while the specificity（69.2%）was significantly higher than that of the latter（61.9%,P〈0.05）.Conclusion：Among patients with ASCUS,HPVE6/E7 mRNA is an effective indicator for colposcopy referral.

关 键 词：人乳头瘤病毒 E6/E7 mRNA 未明确诊断意义的不典型鳞状上皮细胞 灵敏度 特异度 

分 类 号：R737.33[医药卫生—肿瘤]