敌百虫致小鼠外周血淋巴细胞DNA-蛋白质交联作用研究  

Study on DNA-Protein Crosslinks Induced by Trichlorfon in Peripheral Blood Lymphocyte of Mice

作　　者：张勇[1] 何琦 卢黎[1] 杨红[1] 

机构地区：[1]贵州大学动物科学学院高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵阳550025 [2]贵阳市花溪区农业局,贵阳550025

出　　处：《生态毒理学报》2015年第2期210-215,共6页Asian Journal of Ecotoxicology

基　　金：贵州省农业科技攻关项目"绿色富硒猪肉全程质量控制配套技术研究示范项目"[黔科合NY(2007)字(3007)号];贵州省自然科学基金"分子印迹技术应用于猪肉中阿维菌素类药物残留的检测研究"[黔科合J字(2008)2128];贵州大学青年科技基金项目"肉品中农药等有毒有害物质的快速检测及其遗传毒性研究"[贵大自青基合字(2007)074]

摘　　要：为探讨敌百虫致DNA-蛋白质交联作用,以昆明小鼠为受试动物,敌百虫按0、20、40、60 mg·kg-14个剂量水平,灌胃染毒小鼠2周。第5组以提取的正常小鼠外周血淋巴细胞经50μmol·L-1的H2O2处理为阳性对照组。采用经改进的彗星试验方法来检测染毒后外周血淋巴细胞DNA-蛋白质交联效应。结果表明,与空白组相比,各敌百虫染毒组都能引起DNA损伤作用(P<0.01)并引起一定程度的DNA-蛋白质交联效应,在较高浓度(40、60 mg·kg-1)时DNA-蛋白质交联尤为明显,存在一定的潜在突变风险。In order to study DNA-protein crosslinks（DPC） of trichlorfon on peripheral blood lymphocyte in mice, mice were orally administrated with trichlorfon dai/y for 14 days at the concentrations of 0, 20, 40, 60 mg . kg-1 respectively. The fifth group as positive control group was established by extracting the peripheral blood in mice treated with 50 μLmol. L-1hydrogen peroxide. The DPC effects of trichlorfon were measured by comet assay. The results indicated that every group can cause the DNA damaged （P 〈0.01）and lead to DPC effects compared with the blank group. Moreover, DPC is highly obvious when at high concentrations （40, 60 mg. kg-1）, which suggested there was a risk of potential mutation.

关 键 词：敌百虫 小鼠 遗传毒性 DNA-蛋白质交联 DNA断裂 彗星试验 

分 类 号：X171.5[环境科学与工程—环境科学]