溃疡性结肠炎患者肠道不同病变部位Toll样受体4的表达  

Expression of Toll-like receptor 4 in different intestinal lesions of ulcerative colitis patients

作　　者：刘翔[1] 丁元伟[1] 林虹[1] 

机构地区：[1]广州医科大学附属第二医院消化内科,广东广州510260

出　　处：《中国现代医学杂志》2015年第18期38-40,共3页China Journal of Modern Medicine

摘　　要：目的比较溃疡性结肠炎(UC)患者正常肠段和病变肠段中TLR4表达与肠道菌群的差别,探讨TLR4及肠道菌群在UC致病机制中的作用。方法收集2008-2012年广州医科大学附属第二医院肠道病变仅局限于乙状结肠以下的22例UC患者正常肠段和病变肠段的黏膜标本,用荧光定量PCR检测大肠杆菌与乳酸杆菌数量,用Western blot方法半定量检测TLR4的表达。结果 UC患者病变肠段TLR4的表达高于正常肠段,且二者均高于正常人;UC患者病变肠段乳酸杆菌数量低于正常肠段,且二者均低于正常人;UC患者病变肠段大肠杆菌数量高于正常肠段,且二者均高于正常人。结论 TLR4可能参与了UC不同病变部位的致病机制,其表达水平升高可能与肠道菌群失调有关。【Objective】To compare the expression of Toll-like receptor 4（TLR4） and intestine flora in different intestinal lesions of ulcerative colitis（UC） patients and to explore the roles of TLR4 and intestine flora in UC pathogenesis. 【Methods】Twenty-two UC patients with abnormal lesions limited to sigmoid colon in our hospital from2008 to 2012 were selected. Their intestinal mucosa specimens of normal tissues and abnormal lesions were collected. Bacillus acidi lactici and Escherichia coli were quantified by fluorescence quantitative PCR. Western blot was used for semi-quantitative detection of TLR4 on protein level. 【Results】The expression of TLR4 in the abnormal intestinal lesions was higher than that in the normal tissues in the UC patients, and they were both higher than that in normal people. The quantity of Bacillus acidi lactici in the abnormal intestinal lesions was lower than that in the normal tissues in the UC patients, and they were both lower than that in normal people. Quantity of Escherichia coli in the abnormal intestinal lesions was higher than that in the normal tissues in the UC patients, and they were both higher than that in normal people. 【Conclusion】TLR4 may be involved in the pathogenesis of UC, and the elevation of its expression may be correlated with alteration of intestinal flora.

关 键 词：TLR4 肠道菌群 溃疡性结肠炎 致病机制 

分 类 号：R574.1[医药卫生—消化系统]