糖毒性对心肌细胞损伤的研究初探  

Preliminary research into high-glucose induced damage in myocardial cells

作　　者：王庸晋[1] 刘畅[1] 曹文君[1] 郭颖[1] 李丹[1] 赵婷婷[1] 

机构地区：[1]山西省长治医学院血脂代谢与血液病山西省高校重点实验室,046000

出　　处：《国际心血管病杂志》2015年第3期183-185,共3页International Journal of Cardiovascular Disease

基　　金：山西省科学技术发展计划(20130321029-01);长治医学院科技创新项目(CX2014-01)

摘　　要：目的:观察不同葡萄糖浓度下大鼠心肌细胞H9C2的糖代谢变化,初步探讨糖毒性对心肌细胞的损伤作用。方法:以体外培养的大鼠心肌细胞系H9C2为模型,观察不同糖浓度下,心肌细胞的形态学变化,并通过MTT实验检测存活率,流式细胞仪检测H9C2细胞凋亡率。结果:培养24 h后,H9C2细胞形态开始发生变化;培养48 h后,H9C2细胞存活率明显下降、凋亡率上升,且与葡萄糖浓度相关。45 mmol/L葡萄糖刺激心肌细胞48 h后,心肌细胞存活率降至(45.09±0.84)%;持续刺激72 h后,细胞死亡率进一步增高,心肌细胞存活率降至(37.93±0.66)%。15 mmol/L和45 mmol/L葡萄糖处理心肌细胞48 h以上,细胞存活率与对照组相比均显著下降。结论:葡萄糖浓度升高和作用时间延长可促进心肌细胞发生凋亡,高糖对心肌细胞有直接损伤作用。Objective：To study the glucose metabolism state in H9C2 myocardial cell lines under different concentrations of glucose. Methods：H9C2 myocardial cells were cultured in vitro and used as a model system.Morphological changes of myocardial cells were observed under different concentrations of glucose,and the survival rate was measured by MTT assay.Flow cytometry was used to investigate the apoptosis rate. Results：After 24-hour culture under different concentrations of glucose,the myocardial cells began to change in morphology.Forty-eight hours after culture,the survival rate decreased and the apoptosis rate increased,which indicated a dose-dependent property.After stimulation with 45mmol/L glucose for 48 h,the cells survival rate decreased to（45.09±0.84）%,and reached（37.93±0.66）%after stimulation for 72 h.Glucose-stimulation at concentrations of 15mmol/L and 45mmol/L for over48 hboth led to decreased cells survival rate compared with the controls. Conclusion：Apoptosis is induced in myocardial cells with the increasing concentrations and prolonged stimulation of glucose.High glucose causes direct damage to myocardial cells.

关 键 词：糖毒性 H9C2心肌细胞 凋亡 

分 类 号：R363[医药卫生—病理学]