T细胞免疫球蛋白结构域相关分子1在炎症性肠病小鼠不同部位淋巴细胞中表达的变化  

Expression of T cell immunoglobulin and mucin domain-1 in lymphocytes of different tissues in inflammatory bowel disease mice

作　　者：安琦[1] 牛小娟[2] 阎凯[2] 李尧[2] 汪欣[1] 宋京海[2] 

机构地区：[1]北京大学第一医院普通外科,100034 [2]北京大学第五临床医学院卫生部北京医院普通外科

出　　处：《中国医药》2015年第8期1227-1231,共5页China Medicine

基　　金：北京市自然科学基金（7122159）

摘　　要：目的 探讨T细胞免疫球蛋白结构域相关分子（TIM）1在炎症性肠病小鼠不同部位淋巴细胞中表达的变化.方法 将20只雄性BALB/C小鼠完全随机分为观察组与对照组,各10只.观察组饮用4％右旋葡聚糖硫酸钠（DSS）溶液10 d诱发急性结肠炎,对照组小鼠以蒸馏水饲喂10 d.记录2组小鼠的疾病活动指数（DAI）和组织病理学损伤评分,提取外周血、脾脏、肠系膜淋巴结及肠黏膜固有层的淋巴细胞,并予计数,用流式细胞仪检测淋巴细胞的亚群比例及不同部位淋巴细胞中TIM-1表达情况.结果 第10天时,观察组小鼠体质量变化明显小于对照组[（84.8±9.1）％比（114.6±9.3）％],观察组小鼠的DAI、组织病理学损伤评分、脾脏和外周血中淋巴细胞计数明显高于对照组[（11.2±0.8）分比（0.0±0.0）分、（8.8±0.8）分比（0.0±0.0）分、（93.6±21.9）＆#215;106个比（53.3 ±11.9） ＆#215;106个;（5.6±1.2）＆#215;106个比（2.1±0.5）＆#215;106个],差异均有统计学意义（均P＜0.01）,而肠系膜淋巴结中和肠黏膜固有层淋巴细胞计数2组比较差异无统计学意义（P＞0.05）.观察组小鼠脾脏和外周血淋巴细胞中TIM-1＋/CD4＋和TIM-1 ＋/CD8＋细胞比值与对照组比较,差异无统计学意义（P＞0.05）;外周血中TIM-1 ＋/B220＋细胞的比值明显高于对照组,差异有统计学意义[（88.233±0.005）％比（60.164 ±0.109）％,P＜0.05].结论 在DSS诱导的炎症性肠病模型中,急性期脾脏和外周血淋巴细胞的数量均明显升高,TIM-1在急性期阶段外周血中B细胞上表达明显升高.Objective To observe the expression of T cell immunoglobulin and mucin domain-1 （TIM-1） in lymphocytes of different tissues in inflammatory bowel disease （IBD） mice.Methods Twenty male BALB/C mice were randomly divided into observation group and control group.The mice in observation group were treated with dextran sodium sulfate （DSS）（4% DSS contained in drinking water） to induce inflammatory bowel disease （IBD）,and the mice in control group were treated with regular drinking water.The disease activity index （DAI） and histological injury score were analyzed.The lymphocytes extracted from peripheral blood,spleen,mesenteric lymph nodes and intestinal lamina propria were counted and the distribution of lymphocyte subtypes and expression of TIM-1 in lymphocytes were determined by fluorescence activated cell sorter.Results The body weight of the mouse decreased in obseryation group compared with that in control group [（84.8 ± 9.1） % vs （114.6 ± 9.3） %] on the 10th day.The DAI and histological injury score in observation group were significantlyhigher than those in control group [（11.2 ± 0.8） scores vs （0.0 ± 0.0） scores,（8.8 ± 0.8） scores vs （0.0 ± 0.0） scores] （P 〈 0.01）.The countsof lymphocytes from spleen and peripheral blood in observation group were significantly higher than those in control group [（93.6 ± 21.9） ＆#215; 106 vs （53.3 ± 1 1.9） ＆#215; 106,（5.6 ± 1.2） ＆#215; 106 vs （2.1 ± 0.5） ＆#215; 106] （P 〈 0.01）;the countsof lymphocytesfrom mesenteric lymph node and lamina propria showed no significantly differences between the two groups （P 〉 0.05）.The ratio of TIM-1 ＋/CD4＋ in lymphocytes from spleen and peripheral blood were not significantly different in observation group from those in control group （P 〉 0.05）;in lymphocytesfrom peripheral blood,the ratio of TIM-1 ＋/B220＋ in observation group was significantly higher than that in control group [（88.233 ± 0.005） % vs （60.164 ± 0.109） %,P �

关 键 词：炎症性肠病 T细胞免疫球蛋白结构域相关分子1 辅助性T淋巴细胞 

分 类 号：R574[医药卫生—消化系统]