小鼠深低温低流量脑缺血再灌注损伤后miRNA的表达改变  

Expression changes of miRNAs in cerebral ischemia-reperfusion injury after deep hypothermic low flow in mice

作　　者：范长沨 丁培成 张伟艳[1] 温中源 郁迪[1] 王松[1] 莫绪明[1] 

机构地区：[1]南京医科大学附属南京儿童医院心胸外科,210008

出　　处：《国际脑血管病杂志》2015年第5期354-358,共5页International Journal of Cerebrovascular Diseases

摘　　要：目的：观察12种缺血相关性微小RNA（microRNA,miRNA）在小鼠深低温低流量（deep hypothermic low flow , DHLF）脑缺血再灌注后的表达变化。方法3周龄健康清洁级C57BL/6雄性小鼠80只，随机分为DHLF模型组和假手术组，每组再按照再灌注2、6、12和24 h时间点分为4个亚组，每组10只。 DHLF模型组夹闭双侧颈总动脉建立DHLF模型，而假手术组不阻断颈总动脉，在各时间点处死小鼠取脑组织，提取总RN A，应用定量逆转录聚合酶链反应检测m iRN A表达。结果与假手术组比较，DHLF 模型组9种miRNA表达上调，2种表达下调，1种无明显改变。结论11种miRNA表达水平在DHLF后明显发生改变，可能对DHLF后脑缺血再灌注损伤具有调节作用。Objective To observe the expression changes of 12 ischemia-related microRNAs （miRNA） in cerebral ischemia-reperfusion injury after deep hypothermic low flow （DHLF） in mice.Methods A total of 80 3-w eek-old healthy and clean grade C57BL/6 male mice were randomly divided into either a DHLF model group or a sham operation group. Each group was redivided into 4 subgroups according to the time points of 2, 6, 12, and 24 h （10 in each group）. The bilateral carotid arteries of the DHLF model group were clipped and a DHLF model was established, while the carotid arteries of the sham operation group were not clipped. The mice were sacrified at each time point and the brain tissue was removed. The total RNA was extracted. Quantitative reverse transcriptase polymerase chain reaction was used to detect miRNA expression. Results Compared with the sham operation group, the expression levels of 9 miRNAs were upregulated, 2 were dow n-regulated, and 1 did not have any significant change in the DHLF model group. Conclusions The expression levels of 11 miRNAs changed significantly after DHLF. It might have a regulatory role in cerebral ischemia-reperfusion injury after DHLF.

关 键 词：脑缺血 再灌注损伤 微RNAS 低温 人工 小鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]