非霍奇金淋巴瘤Raji细胞照射前后microRNA的差异表达及其功能预测  

Differentially Expressed Micrornas in Non-Hodgkin's Lymphoma Raji Cells Before and After Irradiation and Their Function Prediction

作　　者：伍勇[1] 刘四红[2] 王彩霞[3] 甘庆权[1] 毛平[3] 刘国龙[1] 

机构地区：[1]广州医科大学附属广州市第一人民医院肿瘤科,广东广州510180 [2]广州医科大学附属广州市第一人民医院鹤洞分院,广东广州510380 [3]广州医科大学附属广州市第一人民医院血液科,广东广州510180

出　　处：《临床医学工程》2015年第7期848-851,共4页Clinical Medicine & Engineering

基　　金：国家自然科学基金项目(81201864);广东省医学科研基金项目(B2012301);广州市医药卫生科技项目(201102A213078)

摘　　要：目的寻找调控非霍奇金淋巴瘤放射敏感性的microRNA(miRNAs)。方法利用miRNAs芯片检测非霍奇金淋巴瘤细胞株Raji细胞照射前后miRNAs的表达差异。生物信息学分析这些差异表达的miRNAs的生物学功能。结果用集落形成实验检测Raji细胞的放射敏感性,发现临床常用的分割剂量2 Gy可以导致约50%的生长抑制。因此,给予Raji细胞2 Gy照射。照射后4小时提取RNA用miRNAs芯片进行分析。结果发现,以P<0.05为标准,20个miRNAs照射后有差异表达,其中10个上调,10个下调。实时定量PCR验证部分miRNAs的表达与芯片的结果一致。生物信息学分析表明,大部分差异表达的miRNAs参与细胞周期、凋亡、DNA损伤与修复相关的生物学功能和通路,可能调控Raji细胞的放射敏感性。结论非霍奇金淋巴瘤Raji细胞照射前后miRNAs出现差异表达。差异表达的miRNAs可能参与非霍奇金淋巴瘤放射敏感性的调控。Objective To explore the miRNAs regulating the radiosensitivity of non-Hodgkin＇s lymphoma（NHL）. Methods MiRNA microarray was employed to monitor the expression profiles of miRNAs in NHL Raji cells before and after irradiation. Bioinformatics analysis predicted the biological function of differentially expressed miRNAs. Results The radiosensitivity of Raji cells was evaluated by clonogenic assay. The radiation dose of 2 Gy, which produced about 50% inhibition of growth and was commonly used as fractionation dose clinically,was delivered to Raji cells. The miRNAs expression was assessed at 4 h post irradiation by miRNAs microarray. Mi RNA microarray identified20 miRNAs as differentially expressed： 10 miRNAs up-regulated and 10 down-regulated（defined as P 0.05）. Some of them were verified by real-time quantitative PCR. Bioinformatics analysis showed that most of the differentially expressed miRNAs were involved in cell cycle,apoptosis, DNA damage and repair, and might regulate the radiosensitivity of Raji cells. Conclusions After irradiation, miRNAs are differentially expressed in NHL Raji cells. The differentially expressed miRNAs may be involved in the regulation of NHL radiosensitivity.

关 键 词：非霍奇金淋巴瘤 放射疗法 RAJI细胞 MICRO RNA 

分 类 号：R733.4[医药卫生—肿瘤]