Wnt信号通路抑制剂对人神经胶质瘤细胞增殖活力和生长迁移的影响  

Influence of Wnt signaling pathway inhibitor on proliferation,growth and migration of human glioma cells

作　　者：何东杰[1,2] 胡静[1] 梁军[2] 张洁琼[3] 康蓓佩 骆文静[3] 石梅[1] 魏丽春[1] 

机构地区：[1]第四军医大学西京医院放射治疗科,西安710032 [2]第四军医大学唐都医院放射治疗科,西安710038 [3]第四军医大学劳动与环境卫生教研室,西安710032

出　　处：《神经解剖学杂志》2015年第3期303-308,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81272346;81201749)

摘　　要：目的：探讨Wnt信号通路在胶质瘤细胞增殖和生长迁移中的可能作用。方法：建立U251胶质瘤细胞培养体系、并给予不同浓度Wnt信号通路的抑制剂（IWR-1 0,2.5,5.0,10μmol/L）处理后,MTT测定增殖活力、划痕实验测定生长迁移、Western Blot测定Wnt5a、β-catenin蛋白表达水平,分析IWR-1的生物作用与效应途径。结果：MTT表明胶质瘤细胞的IWR-1 5.0、10μmol/L处理24 h、48 h组,细胞增殖活力显著低于对照组（P〈0.05~0.01）。划痕检测表明IWR-1 5.0、10μmol/L处理48 h组胶质瘤细胞的生长迁移能力低于对照组（P〈0.05~0.01）。Western Blot表明IWR-1处理48 h组胶质瘤细胞的β-catenin蛋白表达水平低于对照组（P〈0.05~0.01）。结论：Wnt信号抑制剂IWR-1能够明显抑制人胶质瘤细胞增殖和生长迁移,提示Wnt/β-catenin信号途径可能具有胶质瘤干预治疗靶点的潜在价值。Objective： To investigate possible involvement of Wnt signaling pathway in cell proliferation and migration of glioma cell. Methods： Cell culture of U251 glioma cell line was established and treated by different concentrations of Wnt signing inhibitor（ inhibitors of Wnt response-1,IWR-1,0,2. 5,5. 0,10 μmol / L）. Cell proliferation was measured by MTT. Cell migration was detected by wound healing. The expression of Wnt5 a and β-catenin proteins was detected by Western Blot. Results： Cell proliferation in U251 glioma cells treated by 5. 0 and 10 μmol / L IWR-1 was lower than that in the control group（ P〈0. 05,P〈0. 01）. The ability of migration in glioma cells treated by 5. 0 and 10 μmol / L IWR-1 was weaker than that of control group at 48 h（ P〈0. 05,P〈0. 01）. The expression of β-catenin protein in glioma cells treated by 5. 0 and 10 μmol / L IWR-1 was lower than that of control group at 48 h（ P〈0. 05,P〈0. 01）. Conclusion： IWR-1 might significantly inhibit cell proliferation,growth and migration of U251 glioma cells,suggesting that Wnt /β-catenin signaling pathway may be a potential intervention target for therapeutic treatment of glioma.

关 键 词：WNT信号通路 胶质瘤细胞 增殖 迁移 

分 类 号：R739.4[医药卫生—肿瘤]