Zebrafish phosvitin is an antioxidant with non-cytotoxic activity  

作　　者：Lili Hu Chen Sun Jing Luan Linlin Lu Shicui Zhang 

机构地区：[1]Laboratory for Evolution and Development, Institute of Evolution and Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 266042, China [2]Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266001, China [3]Center for Reproductive Medicine, Qingdao Women and Children Hospital, Qingdao 266034, China

出　　处：《Acta Biochimica et Biophysica Sinica》2015年第5期349-354,共6页生物化学与生物物理学报（英文版）

摘　　要：Antioxidants, or anti-oxidant agents, have attracted a great deal of attention in recent years because of their roles in prevention of chronic diseases and utilization as preservatives in food and cosmetics. In this study, we clearly demonstrated that zebrafish recombinant phosvitin （rPv） is an antioxidant agent capable of inhibiting the oxidation of the linoleic acid, and scavenging the 2,2-diphenyl-1- picrylhydrazyl radical. We also showed that zebrafish rPv is a cellular antioxidant capable of protecting radical-mediated oxidation of cellular biomolecules. Importantly, zebrafish rPv is non-cytotoxic to murine macrophage RAW264.7 cells. It is the first report that showed the antioxidant activities of Pv in fishes, suggesting that zebrafish Pv can be an important antioxidant, which can be used as preservatives in food and cosmetics and even as supplementary mediator in different diseased states.Antioxidants, or anti-oxidant agents, have attracted a great deal of attention in recent years because of their roles in prevention of chronic diseases and utilization as preservatives in food and cosmetics. In this study, we clearly demonstrated that zebrafish recombinant phosvitin （rPv） is an antioxidant agent capable of inhibiting the oxidation of the linoleic acid, and scavenging the 2,2-diphenyl-1- picrylhydrazyl radical. We also showed that zebrafish rPv is a cellular antioxidant capable of protecting radical-mediated oxidation of cellular biomolecules. Importantly, zebrafish rPv is non-cytotoxic to murine macrophage RAW264.7 cells. It is the first report that showed the antioxidant activities of Pv in fishes, suggesting that zebrafish Pv can be an important antioxidant, which can be used as preservatives in food and cosmetics and even as supplementary mediator in different diseased states.

关 键 词：ZEBRAFISH yolk protein phosvitin （Pv） ANTIOXIDANT OXIDATION 

分 类 号：TS253.1[轻工技术与工程—农产品加工及贮藏工程] TQ464.2[轻工技术与工程—食品科学与工程]