小鼠盲肠系膜三角原位种植结肠癌模型的构建与评价  

Establishment and evaluation of a novel mouse model of orthotopic colon cancer in the mesenteric triangle of the cecum

作　　者：何向锋[1] 施文[2] 文颂[3] 孙永强[2] 凌国杰[4] 沈康[5] 彭春雷[4] 陈宝安[1] 王建红[4] 

机构地区：[1]东南大学医学院附属中大医院血液科,南京210009 [2]南通大学第二附属医院普外科 [3]南昌大学第二附属医院综合介入室 [4]南通市肿瘤医院内科 [5]南通市肿瘤医院科教科

出　　处：《中华肿瘤杂志》2015年第6期418-421,共4页Chinese Journal of Oncology

基　　金：国家重点基础研究发展计划（973计划）子课题（2010CB732404）;国家高技术研究发展计划（863计划）子课题（2012AA022703）;国家自然科学基金（81170492、81370673、81401460）;江苏省科技厅医学重点项目（BL2014078）;中国博士后科学基金项目（2013M530227）;江苏省博士后科研资助计划项目（1301099C）;南通市市级科技计划项目（BK2012067、HS2013030）;南通市卫生局青年基金项目（WQ2014012）

摘　　要：目的：探讨经盲肠系膜三角注射肿瘤细胞悬液制备小鼠结肠癌原位种植模型的可行性。方法手术暴露小鼠盲肠，将小鼠结肠腺癌CT26． WT细胞接种于盲肠系膜三角，建立小鼠结肠癌原位种植模型。结果20只BAL B／c小鼠经盲肠系膜三角注射肿瘤细胞悬液制备小鼠结肠癌原位种植模型的成瘤率为100％，且不易发生梗阻，但可发生肝、脾、横膈、肠系膜淋巴结等多处转移。结论经盲肠系膜三角注射肿瘤细胞悬液制备小鼠结肠癌原位种植模型手术时间短，不易损伤结肠壁，对小鼠的创伤小，手术风险小；手术操作简单，易于掌握。Objective To explore the feasibility of preparation of a mouse model of orthotopic colon cancer by injecting tumor cell suspension into mesenteric triangle of the cecum. Methods Twenty SPF 8-week old BALB/c mice （ male ∶ female=1 ∶ 1） were used in this study. The mouse caecum was exposed by laparostomy, and suspension of mouse colon adenocarcinoma CT26.WT cells was injected into the mesenteric triangle of cecum for preparation of a mouse model oforthotopic colon cancer. Results Mouse orthotopic colon cancer was developed by injection of tumor cell suspension into mesenteric triangle of the cecum showing a successful rate of 100%, without intestinal obstruction, and the liver, spleen, diaphragm and mesenteric lymph nodes metastasis rates were high in all the 20 experimental mice. Conclusions The establishment of mouse models of orthotopic colon cancer by injection of tumor cell suspension into the mesenteric triangle is a simple, rapid, and easy to master procedure, causing less damage to the colon wall, safe and with less trauma to the mice. This method may provide an ideal mouse model of orthotopic colon cancer for the study of pathogenesis as well as liver metastasis mechanisms of colon cancer.

关 键 词：结肠肿瘤 肿瘤转移 肝 小鼠模型 

分 类 号：R735.35[医药卫生—肿瘤] R-332[医药卫生—临床医学]