骨水泥对兔脊柱VX2肿瘤转移模型的作用研究  

The killing effect of bone cement on spinal metastasis of transplanted VX2 carcinoma in experimental rabbit models

作　　者：黄昊[1] 何仕诚[2] 冯国栋[1] 杜瑞杰[1] 朱海东[2] 方文[2] 郭金和[2] 邓钢[2] 

机构地区：[1]东南大学附属中大医院放射科,南京210009 [2]东南大学附属中大医院介入与血管外科,南京210009

出　　处：《介入放射学杂志》2015年第6期520-523,共4页Journal of Interventional Radiology

基　　金：国家自然科学基金(81171434);江苏省临床医学科技专项项目(BL2013029)

摘　　要：目的探讨聚甲基丙烯酸甲酯(PMMA)骨水泥对兔脊柱VX2肿瘤的杀伤作用。方法成功建立兔VX2脊柱转移肿瘤模型18只,随机分为A、B、C组,每组各6只,在CT导下向VX2肿瘤中心分别注入PMMA或生理盐水,其中A组注入PMMA 0.3 ml、B组注入PMMA 0.1 ml、C组注入生理盐水0.3 ml。术后24 h处死模型兔,A、B组分别于距离PMMA团块边缘1、5、10、15 mm处不同方向各取4个肿瘤组织块,C组于瘤体中心至表面不同的4个位置各取1块肿瘤组织,采用脱氧核糖核苷酸末端转移酶介导的缺口末端标记(TUNEL)法检测肿瘤细胞凋亡率。结果 16只模型兔成功注入PMMA骨水泥,A、B组手术成功率均为5/6,C组为6/6,无显著性差异。A组距PMMA边缘1、5、10 mm处肿瘤细胞平均凋亡率分别为(65.75±18.81)%、(50.00±14.24)%、(14.95±8.98)%,与对照组(9.79±5.24)%相比差异均有统计学意义(P<0.05);15 mm处肿瘤细胞平均凋亡率为(10.30±8.13)%,与对照组相比差异无统计学意义。B组距PMMA边缘1、5 mm处肿瘤细胞平均凋亡率分别为(49.20±15.57)%、(17.75±9.28)%,与对照组相比差异均有统计学意义(P<0.05),其余观测点无显著性差异。A、B两组距PMMA边缘1、5、10 mm处肿瘤细胞平均凋亡率比较,差异均有统计学意义(P<0.001)。结论 PMMA可促进肿瘤细胞凋亡,适量增加PMMA注入量可增加肿瘤细胞凋亡范围。Objective To investigate the killing effect of polymethylmethacrylate（PMMA） on spinal metastasis of transplanted VX2 carcinoma in experimental rabbit models. Methods Spinal metastasis of transplanted VX2 carcinoma model was successfully established in 18 rabbits. The experimental rabbits were randomly and equally divided into three groups with 6 rabbits in each group. Under CT guidance, PMMA or saline was injected into the center of VX2 tumor; in group A 0.3 ml of PMMA was used, in group B 0.1 ml of PMMA was used and in group C（control group） 0.3 ml saline was used. Twenty-four hours after the injection, the animals were sacrificed. Four tissue samples were obtained from the sites at 1 mm, 5 mm,10 mm and 15 mm away from the PMMA mass in each rabbit of group A and group B, while four tissue samples were collected from different four sites from the tumor ＇s center to border in each rabbit of group C.Td T-mediated d UTP nick-end labeling（TUNEL） method was used to determine the tumor cell apoptosis rate.Results After successful establishment of rabbit model, injection of PMMA was performed in sixteen among the eighteen rabbits. Technical success rates were 83.3% in both group A and B, and the success rate was100% in group C. The difference in technical success rate was not significant. The mean tumor cell apoptosis rates of spinal VX2 carcinoma at 1 mm, 5 mm and 10 mm away from the PMMA mass in group A were（65.75±18.81）%,（50.00±14.24）% and（14.95±8.98）% respectively. The mean apoptosis rate in the control group was（9.79 ±5.24）%; the differences between the group A and the control group were statistically significant（P 0.05）. The mean tumor cell apoptosis rate of spinal VX2 carcinoma at 15 mm away from the PMMA mass in group A was（10.30 ±8.13）%, which was not significantly different with that of the control group. The mean tumor cell apoptosis rates of spinal VX2 carcinoma at 1 mm and 5 mm away from the PMMA mass in group B were（49.20 ±15.57）% and（17.75 ±9.28）% respe

关 键 词：聚甲基丙烯酸甲酯 兔 VX2肿瘤 脊柱转移 凋亡 

分 类 号：R73-37[医药卫生—肿瘤] R-332[医药卫生—临床医学]