单侧完全性唇裂矫治术后上唇部瘢痕的血管密度及生成模式研究  

Microvascular density and mode of angiogenesis in upper lip scar after surgical repair for unilateral complete cleft lip

作　　者：郑云龙[1] 吕洪 刘洪全[3] 孙坤 关振群 王瑞春 李爱群 

机构地区：[1]青岛大学医学院口腔医学院,266003 [2]青岛市海慈医疗集团病理科 [3]烟台市莱阳中心医院消化内科 [4]青岛市海慈医疗集团口腔科

出　　处：《中华医学美学美容杂志》2015年第3期169-172,共4页Chinese Journal of Medical Aesthetics and Cosmetology

摘　　要：目的 探讨单侧完全性唇裂矫治术后上唇部增生及正常性瘢痕在微血管密度及血管生成模式的差异.方法 2012-2014年,收集单侧完全性唇裂矫治术后上唇部增生性瘢痕标本11例（A组）、正常性瘢痕标本20例（B组）,对收集标本进行常规苏木精-伊红（HE）染色和CD34免疫组织化学染色,对上唇部瘢痕内部结构进行观察,并根据CD34表达对微血管观察计数后计算瘢痕内微血管密度,用Image J软件对血管最大径、最小径及最大径∶最小径比率进行测量分析.结果 上唇部增生性瘢痕（A组）中微血管密度[（87.91士5.95）条/mm2]较上唇部正常瘢痕（B组）中微血管密度[（49.84±7.05）条/mm2]明显增高,差异有统计学意义（P＜0.01）;血管最大径、最小径增生性瘢痕血管分别（38.36±26.36）、（17.33±10.45）μm,正常瘢痕组分别为（13.77±9.56）、（9.00±5.14） μm,差异有统计学意义（P＜0.05）,增生性瘢痕血管最大径和最小径比率为2.85±0.57,明显高于正常瘢痕组的1.91±0.38,差异有统计学意义（P＜0.01）.结论 唇裂矫治术后上唇部瘢痕中微血管密度的明显增高以及血管生成形态和模式的变异可能都与瘢痕的形成及发展有关.Objective To explore the differences between normotrophic and hypertrophic scars of lip after the surgical repair of the unilateral complete cleft lip in density of microvessels and the pattern of angiogenesis.Methods Hypertrophic scars （n=11） and normotrophic scars （n=20） were collected after correction of deformity of the unilateral complete cleft lip,and the tissues were stained with haematoxylin and eosin,and immunostained with anti-CD34 antibody.The structure of scar was observed and the microvessels were counted according to the CD34 expression.Using ImageJ software,the capillary density and length of the major and minor axes were measured,and the major：minor axes ratio was calculated.Results By statistical analysis of the capillary density,the length of the major and minor axes and the major：minor axes ratio were measured;we clarified that there were more capillaries in hypertrophic scars （87.91 ± 5.95）/mm2 than in normotrophic scars （49.84 ± 7.05）/mm2,（P＜0.01）,and the length of the major and minor axes of hypertrophic scars （38.36± 26.36）and （17.33±10.45） μm were longer than the normotrophic scars （13.77±9.56）and （9.00± 5.14） μm,（P＜0.05.） The major：minor axes ratio of hypertrophic scars （2.85±0.57） was higher than the normotrophic scars （2.85 ± 0.57） （P＜0.01）.Conclusions The significant increase in the density of microvessels and the variation in the pattern and morphology of angiogenesis are related to the formation and development of scar after operation of upper lip.

关 键 词：单侧完全性唇裂 上唇瘢痕 增生性瘢痕 血管生成 微血管密度 

分 类 号：R782[医药卫生—口腔医学]