AEG-1、β-catenin和C-myc在原发性肝细胞癌中的临床特性与分析  

Clinical Feature Analysis of AEG-1,β-catenin and C-myc Expression in Primary Hepatocellular Carcinoma

作　　者：程云娟 罗新华[1] 程明亮[1] 

机构地区：[1]贵阳医学院临床医学院感染科,贵州贵阳550001

出　　处：《标记免疫分析与临床》2015年第7期633-636,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：目的了解AEG-1、β-catenin和C-myc在原发性肝细胞癌中的临床特性并进行分析。方法以40例原发性肝癌患者术后肝癌组织作为研究对象;采集肝癌患者的临床及病理学特征,采用免疫组织化学染色及Western Blot方法测定肝癌组织中的AEG-1、β-catenin及C-myc蛋白细胞表达水平及表达载量,分析其表达情况与患者临床及病理特征的关联性。结果 AEG-1在肝癌组织的高表达率为72.5%,以AOD值记分为0.80±0.07,Western Blot测出相对表达量为1.56±0.12;β-catenin蛋白在肝癌组织中高表达率为75.0%,以AOD值记为0.75±0.06,Western Blot测出相对表达量为1.23±0.11;C-myc的高表达率为62.5%,以AOD值记为0.69±0.06、Western Blot测出相对表达量为1.15±0.09。AEG-1、β-catenin和C-myc蛋白在肝癌组织中的表达与肿瘤TNM分期和肝癌组织病理分化程度有关(P<0.05),差异有统计学意义;与患者的性别、年龄、肿瘤大小、HBs Ag、术前AFP浓度均无明显相关(P>0.05),差异无统计学意义。结论 AEG-1、β-catenin和C-myc有望成为评价原发性肝癌恶性程度的指导性指标,进而可以用于评估病程进展及预后;AEG-1、β-catenin和C-myc蛋白在原发性肝癌起病过程中的作用途径独立于AFP系统,为进一步研究原发性肝癌的发病机制提供了新的思路。Objective To investigate the expression level of AEG-1, β-catenin and hepatoeellular carcinoma. Methods 40 cases of primary hepatocellular carcinoma tissue C-myc in primary were used as study samples. The clinical and pathological features of liver cancer patients were recorded. The expression level of AEG-1, β-eatenin and C-myc were determined by using immunohistochemistry and Western Blot method. Results The expression rate of AEG-1, β-catenin protein and C-myc in primary hepatoeellular carcinoma tissues were 72.5%, 75.0% and 62.5% ; the AOD value score were O. 80±0.07, 0.75 ± 0.06 and 0.69 ± 0.06; and the relative expression level was 1.56 ±0. 12, 1. 23 ±0.11 and 1.15 ±0.09, respectively. The expression level of three protein were correlated with pathologic stage differentiation and TNM stage （ p 〈 0.05）. There was no significant correlation with patient＇s gender, age, tumor size, HBsAg, and preoperative AFP oncentration （p 〉 0. 05 ）. Conclusion AEG-1, β-catenin and C-myc might become guidance indexes for nalignant hepatoeellular carcinoma evaluation, and can be used to assess disease progression and prognosis. The role of AEG-1, β-catenin and C-myc protein in the original recurrent hepatocellular carcinoma onsetprocess pathway may be independent from AFP system.

关 键 词：肝癌 AEG-1 Β-CATENIN C-MYC 

分 类 号：R735.7[医药卫生—肿瘤]