下调赖氨酸特异性去甲基酶1表达对于结肠癌LoVo细胞黏附能力的影响  

The effect of decreased expression of lysine specific demethylase 1 on adhesion ability of LoVo cells

作　　者：陈积贤[1] 孙晓雷[2] 张洁[2] 薛迪新[1] 陈澄亮 余铭[1] 林道浙[1] 马艳[3] 林肖[1] 梁美珍[1] 蒋文[4] 

机构地区：[1]温州医学院附属第三医院肿瘤外科,浙江省瑞安325200 [2]南通大学医学院 [3]新疆医科大学公卫教研室 [4]南通市第三人民医院普外科

出　　处：《中华普通外科杂志》2015年第6期476-479,共4页Chinese Journal of General Surgery

基　　金：浙江省自然科学基金资助项目（Y2100917）;南通市科技计划资助项目（BK2013027、HS13921和HS149095）

摘　　要：目的 探讨赖氨酸特异性去甲基酶1 （Lysine specific demethylase 1,LSD1）的表达水平对结肠癌LoVo细胞黏附能力的影响及其分子机制.方法 选取人结肠癌LoVo细胞株,通过LSD1-shRNA质粒瞬时转染人结肠癌LoVo细胞株,RT-PCR和Western blot检测处理后LoVo细胞中KSD1的表达情况,黏附试验检测转染后LoVo细胞的同质性黏附和异质性黏附能力变化,RT-PCR检测处理后LoVo细胞TGF-β1、HES-1、VEGF-C、CD44v6、MMP7、β-catenin等分子的mRNA水平变化.多个样本比较采用单因素方差分析,两两比较采用t检验.结果 LSD1-shRNA质粒瞬时转染人结肠癌LoVo细胞株1h及2h后,细胞的同质性黏附能力均增加（F=151.52和96.47,均P＜0.05）,异质性黏附能力下降（F=87.54和81.50,均P＜0.05）;RT-PCR检测显示VEGF-C、CD44v6、MMP7和β-catenin基因表达水平下降（均P＜0.05）.结论 LSD1下调可以引起LoVo细胞黏附能力改变,过程涉及到β-catenin分子信号通路.Objective To investigate the effect of lysine specific demethylase 1 （LSD1） expression on adhesion ability of LoVo cell and related molecular mechnism.Methods After colon cancer LoVo cell lines being transfected LSD1-shRNA plasmids,RT-PCR and Western blot were used to detect the expression of LSD1;Adhesion test to detect the homogeneity adhesion and heterogeneous adhesion abilities.RT-PCR to detect the mRNA level change of TGF-beta1,hypothesis-1,VEGF-C,CCR7,MMP7 and beta-catenin in LoVo cells.Results LSD1-shRNA plasmids were transfected into LoVo cells after 1 h and 2 h respectively,cell heterogeneous adhesion ability declined （F =151.52,96.47,P ＜ 0.05）.Homogeneity adhesion ability enhanced （F =87.54,81.50,P ＜ 0.05）.The gene expression of VEGF-C,CD44v6,MMP7 and β-catenin decreased obviously （P ＜ 0.05）.Conclusions Downregulted LSD1 expression inhibits colon cancer LoVo cell heterogeneous adhesion abilities and promotes homogeneity adhesion abilities.The mechanism may be related to beta-catenin molecular signal pathway.

关 键 词：结肠肿瘤 细胞增殖 细胞黏附 

分 类 号：R735.34[医药卫生—肿瘤]