重楼皂苷Ⅶ与二氧化硅纳米复合体对卵巢癌耐药性的体外影响  

Effects of Paris polyphylla saponin Ⅶ plus silica nano composite on ovarian cancer drug resistance in vitro

作　　者：杨瑞琦[1] 戚洁[1] 张嘉玲[1] 王飞[1] 范丽梅[1] 

机构地区：[1]吉林大学第二医院妇产科,长春130041

出　　处：《中华医学杂志》2015年第23期1859-1861,共3页National Medical Journal of China

基　　金：中国博士后科学基金（2014T70298）

摘　　要：目的 探讨重楼皂苷Ⅶ联合二氧化硅纳米复合体对于卵巢癌的耐药性.方法 应用MTT法测定重楼皂苷Ⅶ联合二氧化硅纳米复合体对人卵巢癌耐药细胞SKOV3/DDP的增殖抑制率;流式细胞术和Hoechst 33342染色法检测重楼皂苷Ⅶ联合二氧化硅纳米复合体对人卵巢癌耐药细胞SKOV3/DDP的细胞凋亡率及细胞周期时相分布的影响;蛋白免疫印迹法检测凋亡相关蛋白的表达改变.结果 重楼皂苷Ⅶ对SKOV3/DDP细胞的增殖有明显抑制作用,抑制率随药物浓度增加而增高,具有明显的量-效关系（P＜0.05）.重楼皂苷Ⅶ、二氧化硅纳米复合体对SKOV3/DDP细胞作用48、24 h组比较细胞增殖抑制率均明显提高（P＜0.05）.Hoechst 33342染色结果显示,50及100μmol/L的重楼皂苷Ⅶ作用SKOV3/DDP细胞24 h后,不同剂量均能引起细胞出现凋亡的典型表现,即细胞核的深染、凝集和固缩.与阴性对照组比较早期凋亡细胞比例明显增高.蛋白灰度分析结果,与对照组比24 h重楼皂苷Ⅶ处理SKOV3/DDP细胞,50和100 μmol/L剂量组组裂解caspase 3分别增高了5.71倍和11.06倍,Bcl-2表达分别下降了33.3％和61.1％,差异有统计学意义（P＜0.05）.结论 重楼皂苷Ⅶ、二氧化硅纳米复合体对SKOV3/DDP增殖有明显抑制作用,呈明显的浓度依赖性,与时间有相关性,它可引起早期凋亡SKOV3/DDP细胞比例显著增加.Objective To explore the drug resistance of Chonglou saponin Ⅶ plus silica nano complex for ovarian cancer.Methods The method of thiazolyl blue tetrazolium blue （MTT） was employed for determining the proliferation inhibition rate of Chonglou saponin Ⅶ plus silica nano composite for SKOV3/DDP drug-resistant human ovarian cancer cell.Flow cytometry and Hoechst 33342 staining were used for detecting apoptosis rate and cell cycle phase distribution.And the changes of apoptosis-related protein were examined by immunoblot assay.Results There were obvious inhibitory effects of Chonglou saponin Ⅶ on cell proliferation.The inhibition rate increased with rising drug concentration with an obvious dose-effect relationship （P 〈 0.05）.At the same time,the cell proliferation inhibition rate significantly improved at 48 h versus 24 h （P 〈 0.05）.Flow cytometry and Hoechst 33342 staining showed that,after a 24 h treatment of SKOV3/DDP cells with 50 and 100 μmol/L Chonglou saponin Ⅶ,early apoptotic cell ratio significantly increased compared with control group.Conclusion The composite of Paris polyphylla saponin Ⅶ and silica nano has obvious proliferation inhibitory effects on SKOV3/DDP cells.And there is a significant concentration dependence and it also has some correlation with time.The above composite can increased the proportion of early apoptotic cell ratio.

关 键 词：重楼皂苷Ⅶ 人卵巢癌耐药细胞 SKOV3/DDP 细胞增殖 

分 类 号：R737.31[医药卫生—肿瘤]