Oct4、Smad1和Smad2在胃癌中的表达及相关性  

Expression of Oct4, Smad1 and Smad2 in gastric cancer

作　　者：郝美玲[1] 李春辉[1] 

机构地区：[1]承德医学院附属医院病理科,河北省承德市067000

出　　处：《世界华人消化杂志》2015年第16期2539-2548,共10页World Chinese Journal of Digestology

基　　金：河北省政府资助优秀临床人才基金暨河北医学研究重点课题基金资助项目;No.ZL20140103~~

摘　　要：目的:探讨八聚体结合转录因子4蛋白(octamerbinding transcription factor-4,O c t4)与Smad1(drosophila mothers against decapentaplegic protein-1)和Smad2在胃癌和胃正常组织(距癌组织边缘10 cm以上)中的表达及其相互作用.方法:收集60例胃癌(腺癌)组织和25例胃正常组织(距癌组织边缘10 cm以上),应用q RTPCR技术检测Oct4、smad1和smad2基因在组织中的表达情况,应用Western blot蛋白印迹技术检测Oct4、Smad1和Smad2蛋白的表达情况.结果:在胃癌组织中Oct4的表达明显高于胃正常组织(P<0.05),在高、中分化胃癌的表达低于低分化胃癌的表达(P<0.05),O c t 4的表达随肿瘤-淋巴结-转移(tumornode-metasta sis,T NM)分期的升高而增高(P<0.0 5),伴有淋巴结转移的胃癌组织中Oct4较无转移病例表达高(P<0.05);Smad1、S m a d2在正常胃组织表达明显高于胃癌组织(P<0.05),高、中分化胃癌的表达高于低分化胃癌的表达(P<0.05),胃癌组织中S m a d1、S m a d2的表达随T NM分期的升级而降低(P<0.05),伴有淋巴结转移的胃癌组织Smad1、Smad2较无转移病例表达低(P<0.05);上述3个指标与患者年龄及性别均无统计学意义(P>0.05);Oct4与Smad1呈负相关(r=-0.882,P<0.05);Oct4与Smad2呈负相关(r=-0.859,P<0.05);Smad1与Smad2呈正相关(r=0.905,P<0.05).结论:Oct4、Smad1与Smad2的改变与胃癌的发生、发展密切相关.因此,Oct4可能为胃癌的靶向治疗提供新药物作用靶点.AIM：To detect the expression of octamerbinding transcription factor-4（Oct4） and drosophila mothers against decapentaplegic protein（Smad） 1 and 2 in gastric cancer,and to discuss their interactions.METHODS：We collected 60 cases of gastric cancer（adenocardnoma） and 25 cases of normal gastric tissue（above 10 cm from the tumor edge）to detect Oct4,Smadl and Smadl gene expression by quantitative real-time PCR technique and protein expression by Western blot.RESULTS：Oct4 expression was significantly higher in gastric cancer than in normal gastric tissue（P 〈 0.05）.Oct4 expression was significantly lower in well and moderately differentiated gastric cancer than in poorly differentiated gastric cancer（P 〈 0.05）.Oct4 expression increased along with the increase of TNM stage（P 〈 0.05）.Oct4 expression was significantly higher in gastric cancer with lymph node metastasis than in without（P〈 0.05）.Smadl and Smad2 expression was significantly higher in normal gastric tissue than in gastric cancer（P 〈 0.05）.Smadl and Smad2 expression was significantly higher in well and moderately differentiated gastric cancer than in poorly differentiated gastric cancer（P 〈 0.05）.Smadl and Smad2 expression decreased along with the increase of TNM stage（P 〈 0.05）.Smadl and Smad2 expression was significantly lower in gastric cancer with lymph node metastasis than in without（P 〈0.05）.All the above three indicators had no significant correlation with age or gender（P 〉 0.05）;Oct4 expression was correlated negatively with Smadl（r =-0.882,P 〈 0.05）and Smad2 expression（r =-0.859,P 〈 0.05）;Smadl expression was correlated positively with Smad2 expression（r = 0.905,P 〈 0.05）.CONCLUSION：Oct4,Smadl and Smad2 expression may be closely related to the occurrence and development of gastric cancer,and therefore,they may be used as new drug targets for targeted therapy of gastric cancer.

关 键 词：胃癌 OCT4 SMAD1 SMAD2 表达 相互关系 

分 类 号：R735.2[医药卫生—肿瘤]