榄香烯乳对人胰腺癌移植瘤凋亡相关基因表达的影响  

Effect of elemene on expressions of apoptosis-related genes in nude mice with xenografts of human pancreatic carcinoma

作　　者：张莉娜[1,2] 陈恳[1] 胡迎青[1] 钱俐[3] 许丽娜[4] 周国雄[4] 张弘[4] 邵晓轶[1] 贲志云[1] 

机构地区：[1]南通大学医学院免疫系,江苏省226001 [2]南通市第五人民医院 [3]南通大学附属医院肿瘤科 [4]南通大学附属医院消化病科

出　　处：《江苏医药》2015年第12期1387-1389,共3页Jiangsu Medical Journal

基　　金：江苏高校优势学科建设工程资助项目(PAPD);南通大学自然科学课题资助项目(11Z018)

摘　　要：目的探讨榄香烯乳对人胰腺癌移植瘤的抑制作用及其可能机制。方法构建人胰腺癌裸鼠移植瘤模型,随机分为阴性对照组(A组)、小剂量榄香烯乳组(B组)、大剂量榄香烯乳组(C组)、吉西他滨阳性对照组(D组)和联合用药组(E组),每组6只。观察药物干预后肿瘤生长情况,采用实时荧光定量PCR方法检测凋亡相关基因PTEN、p53及Bcl-2的表达情况。结果药物干预15d后,B、C、D、E组肿瘤体积均较A组减小(P<0.01),肿瘤抑制率分别为46.58%、61.77%、74.9%、69.57%。与A组相比,E组PTEN和p53mRNA表达升高(P<0.05),而Bcl-2mRNA表达稍有降低(P>0.05)。结论榄香烯乳对人胰腺癌裸鼠移植瘤有明显抑制作用,可能与PTEN和p53基因表达升高、Bcl-2基因表达降低有关。Objective To investigate the inhibitory effect and underlying mechanism of elemene on the xenografts of human pancreatic carcinoma in nude mice .Methods Thirty nude mice were subcutaneously injected with pancreatic caner cells and then equally randomized into five groups of A（negative control） ,B （injected with elemene 20 mg/kg ） ,C （injected with elemene 60 mg/kg ） , D（injected with gemcitabine 25 mg/kg ） and E （injected with elemene 20 mg/kg plus gemcitabine 25 mg/kg） .The tumor growth was observed ,and mRNA expressions of PTEN ,p53 and Bcl‐2 were detected by fluorescence quantitative‐PCR .Results Compared with group A ,the tumor volume was decreased on the 15th day after treatment in groups of B ,C ,D and E with the inhibition rates of 46.58% ,61.77% ,74.9% and 69.57% ,respectively （P0 .05） .Conclusion Elemene has an inhibitory effect on the xenografts of human pancreatic carcinoma in nude mice ,which may be related to the upregulation of PTEN and p53 expressions and downregulation of Bcl‐2 expression .

关 键 词：榄香烯 胰腺癌 凋亡 

分 类 号：R576[医药卫生—消化系统]