正常前列腺间质细胞对前列腺癌细胞糖代谢水平的影响  

Effects of stromal cells derived from the normal prostate on the glycolysis of prostate cancer cells

作　　者：刘棚越 周娟[1] 彭御冰[1] 王忠[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院泌尿外科,上海200011

出　　处：《中华男科学杂志》2015年第6期489-493,共5页National Journal of Andrology

基　　金：国家自然科学基金(81172450;81202008);上海市卫生局项目(20124Y160);上海交通大学医学院科技基金(13XJ10019)~~

摘　　要：目的:探讨正常前列腺组织不同区带来源的前列腺间质细胞对前列腺癌细胞生长的影响及其作用机制。方法:以激素非依懒性前列腺癌细胞系DU145细胞为研究对象,取新鲜的正常前列腺外周带(PZ)和移行带(TZ)组织,提取间质细胞并体外培养。收集不同区带来源的间质细胞培养上清作为条件培养液培养DU145细胞,CCK8法测定肿瘤细胞的生长曲线,台盼蓝染色测定细胞数量及活力,细胞划痕实验测定细胞侵袭性,Western印迹法测定间质细胞对肿瘤细胞糖代谢关键酶的影响。结果:1PZ间质细胞的条件培养液能促进肿瘤细胞的生长,而TZ间质细胞的条件培养液抑制肿瘤细胞的生长;2PZ间质细胞的条件培养液明显增加肿瘤细胞糖代谢关键酶己糖激酶2(HK-2)、丙酮酸激酶2(PKM-2)、乳酸脱氢酶(LDHA)、丙酮酸脱氢酶(PDH)的表达,而TZ则抑制上述酶的表达。结论:前列腺不同来源的间质细胞对肿瘤细胞的生长影响不同,其机制可能与不同来源的间质细胞对糖酵解代谢的影响不同有关。Objective： To investigate the effects of prostate stromal cells from different zones of normal prostate tissue on the growth of prostate cancer cells and their action mechanisms. Methods： We extracted stromal cells in the fresh normal prostatic tissue derived from the peripheral zone（ PZ） or transitional zone（ TZ）,amplified them in vitro,and used the supernatants of the cells as conditioned media to culture hormone-resistant prostate cancer DU145 cells. We measured the growth curve of the tumor cells using the CCK8 method,determined the number and viability of the cells by trypan blue staining,evaluated their invasiveness by scratch test,and detected the effects of the stromal cells on the key enzymes in the glycolysis of the tumor cells by Western blot. Results： The conditioned medium with the PZ-derived stromal cells promoted,while that with the TZ-derived stromal cells inhibited the growth of the tumor cells. The former significantly increased,while the latter markedly decreased the expressions of the key enzymes hexokinase 2（HK-2）,pyruvate kinase 2（PKM-2）,lactate dehydrogenase（LDHA）,and pyruvate dehydrogenase（PDH） in the glycolysis of the tumor cells. Conclusion： Prostate stromal cells from different zones exert different influences on the growth of tumor cells,which may be associated with their different effects on the glycolysis of tumor cells.

关 键 词：前列腺癌 DU145细胞系 前列腺间质细胞 外周带 移行带 糖酵解 

分 类 号：R737.25[医药卫生—肿瘤]