手机辐射对离体精子质量及DNA甲基化的影响  

Impact of mobile phone radiation on the quality and DNA methylation of human sperm in vitro

作　　者：王东[1] 李博[1] 刘媛[1] 马夜肥[1] 陈书强[1] 孙惠君[1] 董杰[1] 马旭辉[1] 周晶[1] 王晓红[1] 

机构地区：[1]第四军医大学唐都医院生殖中心,陕西西安710038

出　　处：《中华男科学杂志》2015年第6期515-520,共6页National Journal of Andrology

摘　　要：目的:研究手机辐射对男性离体精液的影响及其相关机制。方法:依照WHO《人类精液检查与处理实验室手册》第5版的标准,随机选择97例精液常规参数正常的男性作为研究对象,并将每例研究对象的精液一式2份为对照组和辐射组。辐射组与对照组相比,除了给予一定剂量(1 950 MHz,SAR 3.0 W/kg,时间3 h)的辐射外,其他条件和处理均相同。从精液常规参数、顶体反应能力、精子凋亡程度以及DNA甲基化水平方面,综合分析手机辐射对精液质量的影响。结果:与对照组相比,辐射组前向运动精子百分率显著降低[(36.64±16.93)%vs(27.56±16.92)%,P<0.01],精子活率显著降低[(63.72±16.35)%vs(54.31±17.35)%,P<0.01],精子头部缺陷率显著升高[(69.92±4.46)%vs(71.17±4.89)%,P<0.05],而顶体反应率无统计学差异[(66.20±6.75)%vs(64.50±3.47)%,P>0.05]。与对照组相比,辐射组精子早期凋亡率显著升高[(4.44±5.89)%vs(6.89±9.84)%,P<0.05]。辐射对精子的父源印记基因H19印记基因的印记控制区域及母源印记基因Kv DMR1的DNA甲基化丢失率[(0.60±0.02)%vs(1.40±0.03)%,(0.00±0.00)%vs(1.80±0.03)%,P均>0.05)]均无显著影响。结论:手机辐射可致前向运动精子百分率、存活率显著降低,精子头部缺陷率和精子早期凋亡率显著升高。Objective： To investigate the influences of mobile phone radiation on the quality and DNA methylation of human sperm in vitro. Methods： According to the fifth edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen,we randomly selected 97 male volunteers with normal semen parameters and divided each semen sample from the subjects into two equal parts,one exposed to mobile phone radiation at 1950 M Hz,SAR3. 0 W / kg for 3 hours while the other left untreated as the control. We obtained routine semen parameters as well as the acrosomal reaction ability,apoptosis and DNA methylation of sperm,and compared them between the two groups. Results： Compared with the control,the radiation group showed significantly decreased progressive sperm motility（ [36. 64 ± 16. 93] vs [27. 56 ± 16. 92]%,P 〈 0. 01） and sperm viability（[63. 72 ± 16. 35] vs [54. 31 ±17. 35]%,P 〈 0. 01） and increased sperm head defects（[69. 92 ± 4. 46] vs [71. 17 ± 4. 89]%,P 〈 0. 05）,but no significant differences in sperm acrosomal reaction（[66. 20 ± 6. 75] vs [64. 50 ± 3. 47]%,P 〉 0. 05）. The early apoptosis rate of sperm cells was remarkably higher in the radiation group（[6. 89 ± 9. 84]%） than in the control（[4. 44 ± 5. 89]%）（ P 〈 0. 05）. However,no statistically significant differences were found between the control and radiation groups in the DNA methylation patterns of the paternal imprinting gene H19 ICR（[0. 60 ± 0. 02]vs [1. 40 ± 0. 03]%,P 〉 0. 05） or the maternal imprinting gene Kv DMR1（[0. 00 ± 0. 00]vs [1. 80 ± 0. 03]%,P 〉 0. 05）. Conclusion： Mobile phone radiation reduces the progressive motility and viability of human sperm and increases sperm head defects and early apoptosis of sperm cells.

关 键 词：手机辐射 男性不育 精液参数 顶体反应 精子细胞凋亡 印记基因 DNA甲基化 

分 类 号：R698.2[医药卫生—泌尿科学]