酶法辅助乙醇优选牡丹种皮总黄酮  

Enzyme Assisted Ethanol Extraction of Total Flavonoids from Peony Episperm

作　　者：孟庆焕[1] 祖元刚[1] 王化[2] 王洪政[1] 姜涛 吴薇薇[1] 钟晨[1] 段喜华[1] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040 [2]黑龙江省科学院自然与生态研究所,哈尔滨150040 [3]温州市第二十二中学,温州325003

出　　处：《植物研究》2015年第4期628-631,共4页Bulletin of Botanical Research

基　　金：中央高校基本科研业务费专项资金项目资助DL13BA04;黑龙江省自然科学基金项目(C201235);国家发改委公益性行业科研专项(201404701)

摘　　要：研究适合于以牡丹种皮为原料提取总黄酮的方法,对其提取方法工艺条件进行优化。考察了酶种类、浓度、酶解孵育温度、孵育时间和p H值等因素对提取工艺的影响,并在单因素实验基础上,利用正交实验法进行工艺优化。考察结果表明在果胶酶0.05 mg·m L-1、酶解孵育温度45℃、p H4.5条件下进行酶解孵育180 min后,总黄酮得率最高可达74.839 mg·g-1,相对于乙醇提取法牡丹种皮总黄酮得率显著提高。We optimized enzyme assisted ethanol extraction of total flavonoids from peony episperm. We studied the effects of factors including enzymolysis temperature, enzyme content, enzymolysis pH, enzymolysis time on the extraction ratez of total flavonoids by single-factor and orthogonal test. The optimal extraction conditions were after enzyming pretreatment under the pectinase content of 0.05 mg ·mL-1, enzymolysis temperature of 45 ℃, enzymolysis pH of 4.5, enzymolysis time of 180 rain, total flavonoids yield was up to 74. 839 mg · g-1 Compared with the traditional ethanol extraction, the total flavonoids yield was improved significantly.

关 键 词：牡丹种皮 总黄酮 果胶酶 纤维素酶 

分 类 号：S567.15[农业科学—中草药栽培]