超声法提取山里红叶总黄酮及其抗氧化活性研究  

Optimization of Ultrasonic-assisted Extraction of Total Flavonoids from Crataegus pinnatifida Leaves and Its Antioxidant Activities

作　　者：罗猛[1,2] 宋卓悦 胡娇阳[1,2] 牟璠松[1,2] 乔琪[1,2] 祖元刚[1,2] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040 [2]林下经济资源研发与利用协同创新中心,哈尔滨150040

出　　处：《植物研究》2015年第4期632-637,共6页Bulletin of Botanical Research

基　　金：中央高校基本科研业务费(2572014EY0103;2572014CA08);黑龙江省博士后科研启动金(LBH-Q13003);国家自然科学基金(21403032)

摘　　要：采用超声辅助提取法从山里红叶中提取总黄酮。通过Box-Behnken实验设计(BBD)结合响应面法(RSM)来优化超声提取的条件。影响山里红叶总黄酮提取效率的4个主要变量为液固比,温度,乙醇浓度和时间,得到的最佳值分别为15,40℃,40%,32 min。在此条件下,总黄酮的产率为15.50 mg·g-1。体外抗氧化实验表明,山里红叶提取物的DPPH自由基清除能力为0.69 mg·m L-1(以IC50值表示),与传统的浸渍提取和热回流提取方法相比,超声提取的方法具有更好的抗氧化活性。实验结果表明超声提取法适用于提取山里红叶中的总黄酮,并且其提取物具有较好的抗氧化活性。We used ultrasonic-assisted extraction （UAE） to extract the total flavonoids from Crataegus pinnatifida leaves, and used Box-Behnken design（BBD） combined with response surface methodology （RSM） to optimize extraction conditions. We determined the effects of four variables in terms of ratio of liquid/solid, temperature, ethanol concentration and time on the total flavonoids contents （TFC）. The optimized extraction parameters obtained the highest total flavonoids yield were under the ratio of liquid/solid of 15, 40℃, and 40% of ethanol for 32 min. Under these conditions, the yield was 15.50 mg · g-1 By in vitro antioxidant assays, the extract exhibited a strong DPPH radical-scavenging in ability with IC50 value of 0.69 mg · mL-1. Compared with conventional maceration extraction （ME） and heat-refluxing extraction （HE） methods, UAE method showed remarkable advantages in terms of better antioxidant activities. Overall, the present results indicated that UAE was a suitable method for the extraction of total flavonoids from C. pinnatifida leaves and its extracts were potential antioxidant ingredients.

关 键 词：山里红叶 超声提取 总黄酮 BOX-BEHNKEN设计 抗氧化活性 

分 类 号：Q949.751.8[生物学—植物学]