稻纵卷叶螟羧酸酯酶基因的鉴定与表达谱分析  

Identification and expression profiles of Cnaphalocrocis medinalis carboxylesterase genes

作　　者：刘苏[1] 冯明峰[1] 何梦竹[1] 

机构地区：[1]安徽农业大学植物保护学院,合肥230036

出　　处：《应用昆虫学报》2015年第3期662-670,共9页Chinese Journal of Applied Entomology

基　　金：国家自然科学基金项目(31401734);安徽农业大学引进和稳定人才项目(2013ZR008;YJ2014-2)

摘　　要：【目的】鉴定稻纵卷叶螟Cnaphalocrocis medinalis的羧酸酯酶基因,并检测这些基因在成虫不同组织中的表达模式。【方法】从稻纵卷叶螟转录组中搜索羧酸酯酶基因,使用生物信息学软件对所获得的基因序列进行分析,使用荧光定量PCR检测这些基因在各组织中的相对表达水平。【结果】获得了15个羧酸酯酶基因,分别命名为Cm Car E1~Cm Car E15。其中Cm Car E12缺少3′区域,其余14个序列均含有完整的开放阅读框。除Cm Car E11外,其余14个基因编码的蛋白均具有羧酸酯酶的典型特征,如保守的五肽结构域,催化三联体和氧阴离子穴等。系统进化分析显示15个Cm Car Es被聚在不同的进化支内,Cm Car E13、Cm Car E14和Cm Car E15聚在＂胞内催化类＂进化支,其余12个Cm Car Es聚在＂分泌催化类＂进化支。Cm Car E1、Cm Car E2、Cm Car E3、Cm Car E7、Cm Car E8、Cm Car E10和Cm Car E13特异表达于成虫腹部,而Cm Car E9特异表达于雌雄成虫触角。其他基因的表达没有组织特异性。【结论】Cm Car E1、Cm Car E2、Cm Car E3、Cm Car E7、Cm Car E8、Cm Car E10和Cm Car E13编码的酯酶可能参与了内外源化合物的代谢,而Cm Car E9编码的酯酶可能参与了气味分子的降解。[Objectives] To identify carboxylesterase genes（Car Es） in Cnaphalocrocis medinalis and determine their expression profiles in various adult tissues. [Methods] C. medinalis transcriptome datasets were searched to identify Car Es. Bioinformatic software was used to analyze the identified Car E sequences, and a real-time quantitative PCR assay was performed to investigate their relative expression levels. [Results] A total of 15 Car Es were identified from the C. medinalis transcriptome datasets and named Cm Car E1-Cm Car E15. Of these, Cm Car E12 lacked the 3＇-region whereas the other 14 sequences contained complete open reading frames（ORFs）. With the exception of Cm Car E11, protein encoded by these Cm Car E genes showed features typical of Car Es, including a conserved pentapeptide, catalytic triad, and oxyanion hole. Phylogenetic analysis showed that the 15 Cm Car Es fell into different clades; Cm Car E13, Cm Car E14 and Cm Car E15 fell into the ＂intracellular catalytic class＂, whereas the other 12 Cm Car Es fell into the ＂secreted catalytic class＂. Cm Car E1, Cm Car E2, Cm Car E3, Cm Car E7, Cm Car E8, Cm Car E10 and Cm Car E13 were specifically expressed in the adult abdomen, whereas Cm Car E9 was specifically expressed in male and female antennae. Other genes were not tissue-specific. [Conclusion] Cm Car E1, Cm Car E2, Cm Car E3, Cm Car E7, Cm Car E8, Cm Car E10 and Cm Car E13 might possibly be involved in the metabolism of endobiotic and xenobiotic compounds, whereas Cm Car E9 might be involved in the degradation of odorant molecules.

关 键 词：稻纵卷叶螟 羧酸酯酶 系统进化分析 表达谱 

分 类 号：S435.112.1[农业科学—农业昆虫与害虫防治]