肝细胞生长因子对胃癌细胞增殖、迁移和侵袭的影响  

Effects of hepatocyte growth factor on proliferation, migration and invasion of gastric cancer cell

作　　者：凌跃新[1] 童赛雄[1] 

机构地区：[1]复旦大学附属中山医院普外科,上海200032

出　　处：《外科理论与实践》2015年第3期217-220,共4页Journal of Surgery Concepts & Practice

摘　　要：目的 :探讨肝细胞生长因子(hepatocyte growth factor,HGF)对胃癌细胞株AGS增殖、迁移和侵袭的影响。方法:体外培养AGS细胞,应用浓度为25、50、100 ng/m L的HGF处理AGS细胞,采用CCK-8法检测AGS细胞的增殖,transwell法分别检测HGF对AGS细胞迁移、侵袭能力的影响。结果:CCK-8法检测结果显示,HGF对AGS细胞有促增殖作用。随着HGF浓度的增加或作用时间的延长,细胞增殖活力呈上升趋势,在72 h最为明显,呈时间、浓度依赖关系。Transwell迁移、侵袭实验显示,不同浓度的HGF对AGS细胞的迁移和侵袭能力有促进作用,以50 ng/m L最为明显(P<0.01)。结论 :HGF具有促进胃癌细胞增殖、迁移和侵袭的作用。Objective To explore the effect of hepatocyte growth factor（HGF） on the proliferation, migration and invasion of gastric cancer cell line AGS. Methods Gastric cancer cell line AGS was cultured in vitro. The gastric cancer cells were treated with 25, 50 and 100 ng/m L of HGF. Cell proliferation was measured by CCK-8 assay.Both cell migration and invasion were measured by transwell assay. Results It was shown by CCK- 8 assay that HGF increased the proliferation of AGS cells with different concentrations. HGF increased the proliferation activity of AGS cells in a dose- and time-dependent manner with the peak at 72 h. Transwell assay confirmed that HGF promoted the migration and invasion of AGS cells. Exposure to HGF at 50 ng/m L stimulated the migration and invasion of AGS cells significantly（P〈0.01）. Conclusions HGF might have promotion effect of proliferation, migration and invasion of gastric cancer cells.

关 键 词：肝细胞生长因子 胃癌细胞 增殖 迁移 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]