组织芯片检测凋亡抑制蛋白Livin在前列腺癌中的表达及意义  

Expression and Significance of Livin Protein in Prostate Cancer with Tissue Microarray

作　　者：朱永锋[1] 张春霆[2] 

机构地区：[1]浙江省金华职业技术学院附属金华市人民医院泌尿外科,金华321000 [2]浙江大学金华医院泌尿外科,金华321000

出　　处：《中国中西医结合外科杂志》2015年第3期221-223,共3页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

摘　　要：目的：检测凋亡抑制蛋白Livin在前列腺癌中的表达及其与临床病理特征的关系。方法：采用组织芯片技术构建71例前列腺癌和37例前列腺增生组织的64点阵石蜡组织芯片,用免疫组化SP法检测该芯片中凋亡抑制蛋白Livin的表达,分析其与前列腺癌临床病理特征的关系。结果：凋亡抑制蛋白Livin在前列腺癌组织中的阳性表达率为60.94%,在前列腺增生组织中阳性表达率为2.70%（P〈0.01）。Livin表达与前列腺癌Gleason评分和临床分期有关,Livin在Gleason〈7分的阳性表达率为41.67%,Livin在≥7分的表达率为85.71%,在前列腺癌Ⅰ~Ⅱ期和Ⅲ~Ⅳ期阳性表达率分别为53.66%和82.61%（P〈0.05）。结论：凋亡抑制蛋白Livin表达与前列腺癌的发生进展关系密切。ObjectiveTo detect expression of Apoptosis Inhibitor Protein Livin and clinical pathologicalcharacteristics of patients with prostate cancer.MethodsTissue microarray technology was performed in 71 cases of prostate cancer tissues and 37 cases of benign prostatic hyperplasia, microarray and immunohistochemi-cal method were used to detect expression of Apoptosis Inhibitor Protein Livin in the chip. An important relation-ship was found between the expression of Apoptosis Inhibitor Protein Livin, Gleason score and the clinical stag-es of prostate cancer.ResultsIn patients with prostate cancer, Livin positive expression rate was 60.94%, Inhyperplasia tissue the expression rate was 2.70%, There was a significant difference（P 0.01）. Positive expres-sion rate of Livin in Gleason score 7 was 41.67%, the rate of Livin expression in Gleason score≥7 was 85.71%. The positive expression rates in Ⅰ~Ⅱ and Ⅲ~Ⅳ stages of prostate cancer were respectively 53.66% and 82.61%, and were significantly different（P 0.05）. Livin expression in prostate cancer was significantly re-lated to Gleason score and clinical stage（P 0.05）.ConclusionAbnormal expression of apoptosis inhibitionprotein Livin is closely related to the development of prostate cancer.

关 键 词：前列腺癌 LIVIN 组织芯片 免疫组织化学 

分 类 号：R737.25[医药卫生—肿瘤]