TTF-2在甲状腺转录因子-2转基因小鼠腭突发育过程中的表达  

Expression of TTF-2 in Palate Development of Titf-2 Transgenic Mouse

作　　者：黄磊[1] 倪旭英[1] 石冰[2] 郑谦[2] 蒙田[2] 王龑[2] 

机构地区：[1]广州市第一人民医院口腔科,广东广州510016 [2]口腔疾病国家重点实验室四川大学华西口腔医学院唇腭裂外科,四川成都610041

出　　处：《昆明医科大学学报》2015年第6期26-30,共5页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(30171020);广东省自然科学基金资助项目(S201301001624)

摘　　要：目的探讨甲状腺转录因子-2转基因小鼠继发腭发育过程中,TTF-2 m RNA及蛋白表达的规律.方法于妊娠12 d、13 d、14 d、15 d分别断颈处死各3只孕鼠,无菌条件取出胚胎,解离腭突.应用RT-PCR技术和Western印记方法检测TTF-2在腭突中的表达.结果在甲状腺转录因子-2转基因小鼠胚胎的腭突形成、上抬和融合的发育过程中均有TTF-2的表达,但m RNA及蛋白表达水平变化无统计学意义(P>0.05),RT-PCR检测和Western印记结果一致.结论甲状腺转录因子-2转基因小鼠继发腭发育过程中,TTF-2在腭突中的持续高表达,这可能是甲状腺转录因子-2转基因小鼠出现腭裂的原因之一.Objective The study aimed to investigate the expression of TTF- 2 m RNA in palatal development of Titf- 2 transgenic mouse. Methods From the embryonic day 12（E12） to E15, three pregnant mice were decapitated to obtain the embryos in each day. Palatal shelves were separated from the Titf- 2 transgenic mouse embryos, and the TTF- 2 expression in palatal shelves was determined by RT- PCR and Western blot. Results TTF- 2 was highly expressed in the palatal shelves development of titf- 2 transgenic mouse involving palatal shelves growing, elevating and fusing, but there was no significant difference in the change of expression between m RNA and protein. The result of RT- PCR test was consistent with that of Western blot. Conclusion TTF- 2 is highly expressed in the palatal shelves from the embryonic day 12（E12） to E15 in Titf- 2 transgenic mice, and the overexpression of TTF- 2 during palatogenesis may contribute to the formation of cleft palate.

关 键 词：TTF-2 转基因小鼠 腭突 

分 类 号：R782.22[医药卫生—口腔医学]