硫酸阿托品滴眼液滴眼和氢溴酸东莨菪碱皮下注射制作水液缺乏型干眼兔模型实验研究  

Research on the atropine sulfate eye drops and scopolamine hydrobromide subcutaneous injection production water lack of rabbit's dry eye model

作　　者：刘雪[1] 徐雯[1] 高卫萍[1] 

机构地区：[1]南京中医药大学第一临床学院,南京210029

出　　处：《临床眼科杂志》2015年第3期263-266,共4页Journal of Clinical Ophthalmology

基　　金：江苏省自然科学基金(BK2012856);国家自然科学基金(81373746)

摘　　要：目的比较硫酸阿托品滴眼液滴眼和氢溴酸东莨菪碱皮下注射制作水液缺乏型干眼兔模型各自的特点。方法健康新西兰大白兔18只,随机分为正常组、阿托品组、东莨菪碱组3组。正常组6只兔不接受任何处理;阿托品组6只兔双眼滴1%硫酸阿托品滴眼液;东莨菪碱组6只兔皮下注射氢溴酸东莨菪碱注射剂。3组分别于实验前及实验第3、7、21、28天检查Schirmer I试验、泪膜破裂时间、角膜荧光素染色。结果东莨菪碱组在实验第7、14、21、28天,Schirmer I试验、泪膜破裂时间和角膜荧光染色3项检查与实验前相比(P<0.05),具有明显差异;阿托品组在实验第3、7、14天,3项检查与实验前相比(P<0.05),差异具有意义,且第7天差异达到最大,至实验第21天时,P>0.05,无明显差异;实验第3天东莨菪碱组比阿托品组相比(P<0.05),差异具有显著意义;实验第21天东莨菪碱组比阿托品组(P<0.05),差异具有义。泪腺病理学检查东莨菪碱组较阿托品组明显有大片炎细胞浸润,腺上皮细胞萎缩。结论硫酸阿托品滴眼液点眼制作的干眼模型经济、便捷、起效快,但不能持久;氢溴酸东莨菪碱注射剂皮下注射制作干眼模型起效较慢,但稳定长久,并对泪腺上皮细胞影响明显。Objective To compare atropine sulfate eye drops and subcutaneous scopolamine hydrobromide pro-duced aqueous-deficient dry eye rabbit model of their own characteristics. Methods 18 healthy New Zealand white rab-bits were randomly divided into normal group,the atropine group,scopolamine group three groups. Normal group six rab-bits do not accept any treatment;atropine group six rabbits eye drops of 1% atropine sulfate eye drops;scopolamine group six rabbits subcutaneous injections of scopolamine hydrobromide. 3 groups were examined before the experiment and the ex-periment on day 3,7,21,28 Schirmer I test,tear film break-up time,corneal fluorescein staining. Results Scopolamine 7,14,21 and 28 days in the experimental group,Schirmer I test,tear film break-up time and corneal staining compared with the experimental examination 3 （P 〈 0. 05）,with a significant difference;atropine group in the first experiment 3,7, 14 days,compared with the previous three checks experiment （P 〈 0. 05）,meaning the difference,and the first seven days difference between the maximum,to test the first 21 days,P ＆gt; 0. 05,no significant difference;On experiment 3 day compared scopolamine group than atropine group （P 〈 0. 05）,the difference was significant;the first 21 days the experi-mental group than in the scopolamine atropine group （P 〈 0. 05）,the difference was righteous. Lacrimal gland pathology scopolamine group was there a large group of inflammatory cell infiltration compared with atropine,glandular epithelium at-rophy. Conclusions Atropine sulfate eye drops for dry eye model produced economic,convenient,rapid onset,but can not be sustained;scopolamine hydrobromide from subcutaneous injections produced slow onset of dry eye model,but long-term stability,and the impact of the lacrimal gland epithelial cells obvious.

关 键 词：阿托品 东莨菪碱 干眼 动物模型 

分 类 号：R-332[医药卫生] R777.34