结直肠腺瘤中IGF-Ⅰ、IGF-ⅠR、IGF-Ⅱ的表达及其意义  

Meaning of IGF-Ⅰ,IGF-ⅠR and IGF-Ⅱexpression in colorectal adenoma

作　　者：谭晓林[1] 米贤军[2] 刘祥玉[2] 尹泉[1] 谢彩虹[1] 赵超[1] 陈冬妹[1] 肖文[1] 施为建[1] 

机构地区：[1]南方医科大学附属中山市博爱医院消化内科,广东中山528403 [2]南方医科大学附属中山市博爱医院病理科,广东中山528403

出　　处：《临床消化病杂志》2015年第3期157-162,共6页Chinese Journal of Clinical Gastroenterology

基　　金：中山市科技局基金资助项目(No:20113A124)

摘　　要：[目的]探讨胰岛素样生长因子(insulin-like growth factor,IGF)-Ⅰ、IGF-ⅠR、IGF-Ⅱ在结直肠腺瘤(colorectal adenoma,CRA)中的表达及意义。[方法]入选行电子结肠镜检查并经活检病理确诊的CRA 100例、结直肠癌(colorectal cancer,CRC)30例、炎性息肉30例,另选20例正常结直肠黏膜者作为阴性对照,应用免疫组织化学SP法检测所有入选者IGF-Ⅰ、IGF-IR、IGF-Ⅱ的表达。[结果]阴性对照组IGF-Ⅰ、IGF-ⅠR、IGF-Ⅱ的阳性表达分别为5.0%、10.0%、10.0%,与炎性息肉组(分别为6.7%、10.0%、10.0%)差异无统计学意义(P>0.05),CRA组(分别为36.0%、48.0%、44.0%)、CRC组(分别为63.3%、80.0%、73.3%)较阴性对照组、炎性息肉组均呈逐渐增加趋势,差异有统计学意义(P<0.01);CRA组IGF-Ⅰ、IGF-ⅠR、IGF-Ⅱ的阳性表达与性别、年龄、数量、部位均无明显关系(P>0.05),与腺瘤大小、增生程度、组织学分类差异有统计学意义(P<0.01);CRA组IGF-Ⅰ、IGF-ⅠR、IGF-Ⅱ的表达相互间呈正相关(P<0.01)。[结论]在正常人群的结直肠黏膜向腺瘤的转化,以及腺瘤的增长和转化过程中,IGFs和IGFs-R轴发挥着极其重要作用,IGF-Ⅰ、IGF-ⅠR、IGF-Ⅱ有可能作为CRA癌变的预测指标,对临床早期诊治具有重要意义。[Objective]To explore the meaning of insulin-like growth factor（IGF）-Ⅰ,IGF-Ⅰ receptor （IGF-ⅠR）and IGF-Ⅱ expression in colorectal adenoma. [Methods]Specimens were collected from 100 patients with colorectal adenoma（CRA）,30 patients with colorectal cancer（CRC）and 30 patients with inflammatory polyps who underwent colonoscopy and pathological biopsy at Boai hospital from January 2011 to June 2013. Twenty cases of normal colorectal mucosa were used as a negative control group. Expression of IGF-Ⅰ,IGF-ⅠR, and IGF-Ⅱ in the specimens were detected by immunohistochemical SP method. [Results]There were no significant differences in the positive rates of IGF-Ⅰ ,IGF-ⅠR and IGF-Ⅱ expression between the normal colorectal mucosa group and inflammatory polyps group（5.0% vs 6.7%,10.0% vs 10.0%,10.0% vs 10.0%,P〉 0.05 for all）;however,significant differences were observed in the normal colorectal mucosa group and inflammatory polyps group when compared with the CRA group（36.0% ,48.0%,44.0%）and CRC group（63.%,80.0%,73.3%） （P〈 0.01 for all）. In the CRA group, the positive expression of IGF- Ⅰ,IGF- ⅠR and IGF-Ⅱ were significantly correlated with adenoma size,hyperplastic degree and histological classification（P〈 0.01 for all）, but not with gender, age, number, or site of the adenoma（P〉 0.05 for all）. In the CRA group,there were positive correlations between the expression of IGF- Ⅰ,IGF- ⅠR and IGF-Ⅱ （P〈 0.01 for all）. [Conclusion]The IGFs/IGFs-R axis plays an important role in the transformation of normal colorectal mucosa to adenoma and in growth and transformation of the adenoma. IGF-Ⅰ,IGF-ⅠR and IGF-Ⅱ can be used as predictive indices for malignant transformation of colorectal adenoma.

关 键 词：结直肠腺瘤 胰岛素样生长因子Ⅰ 胰岛素样生长因子Ⅱ 胰岛素样生长因子Ⅰ受体 免疫组织化学 

分 类 号：R735.34[医药卫生—肿瘤]