人急性B淋巴细胞白血病-NOD/SCID小鼠模型的建立  

Establishment of Human Acute B-Lymphoblastic Leukemia-NOD/SCID Xenotransplant Mouse Model

作　　者：张欣[1] 邓媛[1] 陈凤丽[1] 房宜嘉 张伟[2] 于亮[1] 

机构地区：[1]南京医科大学附属淮安第一医院血液科,江苏淮安223300 [2]淮安市食品药品检验所,江苏淮安223300

出　　处：《中国实验血液学杂志》2015年第3期623-626,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目(81170489);江苏省自然科学基金资助项目(BK2010287;BK20141254);江苏省卫生厅基金资助项目(H201056)

摘　　要：目的:应用NOD/SCID小鼠构建人急性B淋巴细胞白血病-NOD/SCID小鼠模型。方法:采用4-5周龄NOD/SCID小鼠,每只小鼠尾静脉注射5×106个Nalm-6细胞,通过对小鼠一般状态,骨髓涂片,组织病理学检查等方法对白血病模型进行鉴定。结果:注射白血病细胞15 d后,小鼠开始出现精神萎靡、食欲不振、皮毛皱缩、后肢无力和脊柱抬高等表现,骨髓涂片可见成团分布的白血病细胞,脾脏组织病理切片亦可见白血病细胞浸润。结论:通过尾静脉注射Nalm-6细胞于未经照射的NOD/SCID小鼠中可以成功建立全身播散的白血病模型,该模型的构建操作简单易行,成功率高,重复性好,为研究白血病发病机制及指导临床化疗方案设计提供了良好的研究平台。Objective： To establish human B-acute lymphoblastic leukemia NOD/SCID mouse model. Methods： The unirradiated mice aged 4 -5 weeks were injected with Nalm-6 cells via the tail vein. Successful transplantation was assessed by the general state, bone marrow smear and histopathologic examination. Results：After 15 days of injection with Nalm-6 cells, the NOD/SCID mice displayed the morbidily with lethargy, hind limb paralysis, hunched back and lossing weight. The infiltration of leukemia cells could be observed in bone marrow and spleen sections. Conclusion： Systemic B lineage acute leukemia animal model has been successfully established in the NOD/SCID mice, which is a useful tool for studying pathogenesis of leukemia and guiding clinical chemotherapy regimens.

关 键 词：急性B淋巴细胞白血病 NOD/SCID 动物模型 

分 类 号：R733.71[医药卫生—肿瘤]