盐霉素增强长春新碱诱导急性T淋巴细胞白血病Jurkat细胞株凋亡研究  

Salinomycin Enhances the Apoptosis of T-cell Acute Lymphoblastic Leukemia Cell Line Jurkat Cells Induced by Vincristine

作　　者：刘平平[1,2] 朱锦灿[3] 刘革修[3] 税朝祥[1] 李小梅[1] 

机构地区：[1]清华大学医学中心,北京100084 [2]清华大学第一附属医院血液肿瘤科,北京100016 [3]暨南大学医学院血液病研究所,广东广州510632

出　　处：《中国实验血液学杂志》2015年第3期653-657,共5页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目(81270568)

摘　　要：目的:本文旨在研究长春新碱(vincristine,VCR)与盐霉素(salinomycin,Sal)联合作用对急性T淋巴细胞白血病jurkat细胞株增殖、凋亡的影响及其可能的机制。方法:CCK-8法检测细胞增殖情况;流式细胞术检测各实验组细胞凋亡率;Western blot检测凋亡相关蛋白BCL-2、caspase-3和caspase-8表达水平。结果:VCR、Sal单独及联合处理Jurkat细胞株均显示出明显的增殖抑制作用,两药联合使用的增殖抑制作用更显著(P<0.05),具有协同作用。联合用药组BCL-2蛋白水平较VCR和Sal单独处理组明显减少,而caspase-3和caspase-8水平明显增高;VCR和Sal联合用药组细胞凋亡率较VCR和Sal单独处理组明显提高(P<0.05)。结论:长春新碱联合盐霉素作用于Jurkat细胞具有协同抑制增殖及诱导凋亡的作用。Objective： This study was aimed to investigate the effect of salinomycin combined with vincristine on the proliferation and apoptosis of Jurkat cells and its possible mechanisms. Methods ： The proliferation of Jurkat cells was examined by CKK-8 assay. Flow cytometry was used to assess cellular apoptosis. Levels of BCL-2, caspase-3, and caspase- 8 were measured by Western blot. Results： The salinomycin or vincristine, either alone or in combination, inhibited the proliferation of Jurkat cells in a dose-dependent manner. Salinomycin combined with vincristine produced more obveous inhibition of cell proliferation than either compound used alone （ P 〈 0.05 ）. Western blot analysis showed that the combined use of Sal and VCR reduced the expression of BCL-2 protein, and increased expression of caspase 3 and caspase 8 protein, more significantly. Furthermore, combination of Sal and VCR synergistally promoted apoptosis of the Jurkat cells （P 〈 0.05 ）. Conclusion： The combination of salinomycin and vincristine synergistically inhibits proliferation and promotes apoptosis of T-cell acute lymphoblastic leukemia Jurkat cells.

关 键 词：长春新碱 盐霉素 急性T细胞白血病 JURKAT细胞株 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤]