nCD64对化疗后血液肿瘤患者细菌感染的诊断意义  

Diagnostic Value of Neutrophil CD64 for Bacterial Infection in Patients with Hematologic Malignancies after Chemotherapy

作　　者：史晋叔 张娟[1] 吴琼[1] 李剑[1] 

机构地区：[1]南昌大学第二附属医院血液内科,江西南昌330006

出　　处：《中国实验血液学杂志》2015年第3期852-855,共4页Journal of Experimental Hematology

基　　金：江西省科技支撑计划项目(20122BBG70107-1);江西省卫生厅重大招标项目(20114006)

摘　　要：目的:探讨化疗后的血液肿瘤患者外周血中中性粒细胞CD64(neutrophil CD64,n CD64)平均荧光强度对细菌感染的诊断意义。方法:采用流式细胞术测定研究对象外周血n CD64平均荧光强度,并测定部分研究对象的降钙素原(PCT)及C-反应蛋白(CRP)值作为对照;通过连续动态监测部分血液肿瘤患者外周血n CD64平均荧光强度,分析其与细菌感染之间的关系。结果:n CD64的表达不受中性粒细胞数量的影响(P>0.01),感染组、动态监测组n CD64平均荧光强度及PCT、CRP值均明显高于未感染组(P<0.01);n CD64平均荧光强度诊断细菌感染的敏感性及特异性高于PCT及CRP。结论:n CD64平均荧光强度可作为化疗后血液肿瘤患者细菌感染的诊断指标,并可在一定程度上预测细菌感染。Objective： To investigate the value of CD64 mean fluorescence intensity of the peripheral blood neutro- phils as a diagnostic marker of bacterial infection in patients with hematologic malignancies after chemotherapy. Methods： The neutrophil CD64 mean fluorescence intensity of all patients was detected by flow cytometry, and compared with procalcitonin（PCT） and C reactive protein（CRP） detected in part of patients; the relationship between nCD64 and bacterial infection were analyzed through continuous dynamic monitoring nCD64 mean fluorescence intensity in part of patients. Results： The expression of nCD64 was not affected by neutrophils counts （ P 〉 0.01 ） ; the nCD64 mean fluorescence intensity, PCT and CRP levels in infection group and dynamic monitoring group were significantly higher than those in non-infected group （ P 〈 0.01 ） ; the sensitivity and specificity of nCD64 mean fluorescence intensity were much higher, as compared with PCT and CRP in diagnosis of bacterial infection. Conclusion： nCD64 mean fluorescence intensity can be used as an effective diagnostic marker for bacterial infection in patients with hematologic malignancies after chemotherapy, and may be used to forecast bacterial infection to a certain extent.

关 键 词：n CD64平均荧光强度 细菌感染 化疗 血液肿瘤 

分 类 号：R733[医药卫生—肿瘤] R378[医药卫生—临床医学]