微生物纤维素食品“椰果”毒理学安全性评价  

Toxicological assessment of microbial cellulose food of Nata de coco

作　　者：冯礼明[1] 黄业宇[1] 郑定仙[1] 冯丁山[1] 林卫华[1] 马永忠[1] 

机构地区：[1]海南省疾病预防控制中心,海南海口570203

出　　处：《中国热带医学》2015年第6期651-654,共4页China Tropical Medicine

基　　金：海南省自然科学基金项目(No.30328)

摘　　要：目的评价椰果的安全性,为微生物纤维素食品的开发提供依据。方法椰果脱水制成干粉后按GB15193-2003规定的方法进行小鼠急性经口毒性试验、遗传毒性试验(Ames试验、小鼠骨髓微核试验、小鼠精子畸形试验)、30d喂养试验及致畸试验。结果小鼠急性经口毒性MTD大于15g/kg·bw,三项遗传毒性试验结果均为阴性,30d喂养试验大鼠的生长发育、脏体比、组织病理学、血液和生化指标无异常变化,最大无损害作用剂量估算大于5.0 g/kg·bw,为人体推荐摄入量的100倍,致畸试验对孕鼠无明显的母体毒性,对胎鼠亦无明显胚胎毒性和致畸性。结论椰果干粉属无毒级和无遗传毒性物,在本实验剂量范围内属安全受试物,验证了椰果的食品安全性。Objective To evaluate the safety of Nata de coco and to provide the basis for the development of microbial cellulose food. Methods Nata de coco was dehydrated into powder and acute toxicity test, genetic toxicity test（Ames test, bone marrow cell micronucleus test and mice sperm abnor mality test）, thirty-days feeding test in rats, teratogenicity study were conducted according to GB 15193-2003. Results The acute oral MTD in mice was over 15.0 g/kg· bw. The results of ree genetic toxicity tests were all negative. During thirty-days feeding test, the rats in experimental groups were in good state and no abnormal changes in hematology, biochemistry, viscera modulus and histology were observed. No-observed-adverse- effect-level（NOAEL） of Nata de coco powder on the rats was 5.0 g/kg. bw that was equivalent to 100 times of the recommended dosage for human body. No significant maternal toxicity, teratogenicity and mutagenicity to the pregnant rats and embryos were observed. Conclusion The Nata de coco powder is a non-toxic grade material and no genotoxicity. It is a safe food in the range of experimental doses,food safety of Nata de coco has been verified.

关 键 词：椰果 微生物纤维素 毒理学试验 食品安全性评价 

分 类 号：R285.1[医药卫生—中药学]